Bai / Sáez Gap Junction Channels and Hemichannels

Erscheinungsjahr 2016
ISBN: 978-1-4987-3863-7
Verlag: CRC Press
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 305 Seiten

Reihe: Methods in Signal Transduction Series

Gap junction channels are a group of intercellular channels expressed in tissues and organs to synchronize many physiological processes. Pannexin channels are a family of closely related large-pore channels virtually unknown a decade ago. The field of connexin and pannexin research has recently exploded and became one of the most active areas of research. Numerous novel approaches and techniques have been developed, but there is no single book to dedicate on the unique techniques and protocols for the research on these large pore channels. The authors plan a book to focus on recent state-of-the-art techniques and protocols in various aspects of this diverse research field.

Autoren/Hrsg.

Bai, Donglin

Sáez, Juan C.

Fachgebiete

• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

Gap junction channels. Bioinformatics analysis of connexin genes. Structural approaches on gap junction channels. Fluorescent label of connexins. Functional study of gap junction channel gating. Quantitative measurement of dye transfer. Permeability. Pharmacology of gap junction channels. Gap junction mimetic piptides. Gap junction hemichannels. Dye uptake and ATP release. Functional study of hemichannel current. Connexin purification and reconstitution in lipid bilayers. Hemichannel function in the ovary. Pannexin channels. Functional study pannexin channels. Pannexin channels in vasculature. Pharmacology of pannexin channels. Dye uptake and ATP release.

Über Autor(innen)

Donglin Bai received his Ph.D. from University of Cambridge, U.K. (1994). He then moved to Canada and worked on neurotransmitter receptors (GABA, NMDA, AMPA, noradrenaline, angiotensin) in Loeb Research Institute (Ottawa), Department of Physiology (Toronto) and Samuel Lunenfeld Research Institute (Toronto) as a postdoctoral fellow. In 2002 he was recruited as an Assistant Professor at Department of Physiology and Pharmacology, the University of Western Ontario. He is currently a tenured Associate Professor in the same university. His current research interests are on the physiology of gap junction channels, including gap junction channel docking, single channel conductance and gating properties. He is also interested in revealing how gap junction gene mutants linking to human diseases (cardiac arrhythmias, hypomyelination, cataracts, deafness and skin diseases) and developing strategies of rescuing the mutants. He received grants support from the following Canadian funding agencies: CIHR, CRC, NSERC, HSFC and ERA.