Becher Microbial Proteomics

Methods and Protocols
1. Auflage 2018
ISBN: 978-1-4939-8695-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1841, 344 Seiten, eBook

Reihe: Methods in Molecular Biology

This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractionation, protein quantification, analysis of post-translational protein modifications, as well as metaproteomics, a relatively new branch of microbial proteomics that investigates the proteins of all microbes comprising an environmental consortium. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tipson troubleshooting and avoiding known pitfalls.  Authoritative and practical, Microbial Proteomics: Methods and Protocols serves as a valuable and stimulating source for all beginners and advanced researchers in the field of microbial proteomics and beyond. Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Becher, Dörte

Inhaltsverzeichnis

Filter-Aided Sample Preparation for Proteome Analysis.- Protein Enrichment from Highly Dilute Samples with StrataClean.- Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins.- Enrichment of Cell Surface-Associated Proteins in Gram + Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis.- Preparation of Bacterial Magnetosomes for Proteome Analysis.- Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics.- Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS.- Applications of Difference Gel Electrophoresis (DIGE) on the Study of Microorganisms.- Proteomic Signatures in Staphylococcus aureus.-  How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in Bacillus subtilis.-  Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE.- Sulfur- 34 S and 36 S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses.- Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes ( 15 N).- Next-Generation Trapping of Protease Substrates by Label-Free Proteomics.- In Vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host-Pathogen Settings.- Phosphopeptide Enrichment from Bacterial Samples Utilizing Titanium Oxide Affinity Chromatography.- Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation.- Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria.- Sequential Isolation of DNA, RNA, Protein, and Metabolite Fractions from Murine Organs and Intestinal Contents for Integrated Omics of Host-Microbiota Interactions.- Utilization of a Detergent-Based Method for Direct Microbial Cellular Lysis / Proteome Extraction from Soil Samples for Metaproteomics Studies.- Sample Preparation for Metaproteome Analyses of Soil and Leaf Litter.- Centrifugation-Based Enrichment of Bacterial Cell Populations for Metaproteomic Studies on Bacteria-Invertebrate Symbioses.