Biancotto / Dagur Immunophenotyping

Methods and Protocols
2. Auflage 2027
ISBN: 978-1-0716-5607-5
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 484 Seiten

Reihe: Springer Protocols

This new edition reflects the transformative developments in immunophenotyping research while maintaining a strong foundation in established methodologies essential for both research and clinical applications. Beginning with chapters involving fundamental immune cell populations, the book continues by exploring clinical applications in hematological malignancies and cellular therapies, as well as methodological advances and analytical frameworks essential for modern immunophenotyping. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Practical and up-to-date, aims to provide researchers and clinicians with representative protocols spanning fundamental techniques to cutting-edge technologies.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Biancotto, Angélique

Dagur, Pradeep K.

Inhaltsverzeichnis

Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T-Cells.- Enumeration of Benign and Malignant Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis.- Neutrophil Subset Immunophenotyping.- Natural Killer Cells and Innate Lymphoid Cells Immunoprofiling.- Immunophenotyping of Human Regulatory T-Cells from Peripheral Blood Mononuclear Cells.- Immunophenotyping of Human B Lymphocytes from Peripheral Blood Mononuclear Cells.- Immunophenotyping Studies in Non-Human Primate Models.- Immunophenotypic Assessment of Natural Killer Cell Degranulation and Monocyte Function from Cryopreserved Peripheral Blood Mononuclear Cells.- Immunophenotyping of Acute Myeloid Leukemia.- Flow Cytometric Immunophenotyping of Acute Lymphoblastic Leukemia.- Clinical Flow Cytometric Testing in Chronic Lymphocytic Leukemia.- Measurable Residual Disease.- Immunophenotyping of Cellular Therapy Products Against Oncogenic and Viral Targets.- Guidelines for Gating Flow Cytometry Data for Immunological Assays.- Quality Control of Immunophenotyping.- High Immunophenotyping of Human PBMCs Using Lyophilized Antibodies.- Immunophenotyping by Single Cell CITE-Seq.- Immunophenotyping by Spatial Biology.- High Dimensional Immunophenotyping of Human Leukocytes by Spectral Cytometry.- Build Heatmaps of Cell Frequencies and Marker Intensities for Correct Interpretations.- High-Dimensional Multimodal Data Integration for Immune-Driven Diseases: A Practical Guide.