E-Book, Englisch, 205 Seiten, Web PDF
Block / Le Strange / Zweig Paper Chromatography
1. Auflage 2013
ISBN: 978-1-4832-7687-8
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark
A Laboratory Manual
E-Book, Englisch, 205 Seiten, Web PDF
ISBN: 978-1-4832-7687-8
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark
Paper Chromatography: A Laboratory Manual focuses on methods, technologies, and processes, and aims to provide readers with a readily accessible source for the uses and adaptations of paper chromatography. The book first offers information on general methods, including descending, ascending, and ascending-descending chromatography, filter paper ''chromatopile'', ''reversed phase'' paper chromatography, and paper electrophoresis. The text then elaborates on quantitative methods and amino acids, amines, and proteins. Discussions focus on visual comparison, elution, area of spot, total color of spot, maximum color density, identification of amines, separation of proteins, and general directions. The publication examines carbohydrates and aliphatic acids and steroids. Topics include simple sugars, miscellaneous derived sugars, and aliphatic acids. The text also ponders on purines, pyrimidines, and related substances and phenols, aromatic acids, and porphyrins. The text is a valuable reference for readers interested in paper chromatography.
Autoren/Hrsg.
Weitere Infos & Material
1;Front Cover;1
2;Paper Chromatography: A Laboratory Manual;4
3;Copyright Page;5
4;Table of Contents;8
5;FOREWORD;6
6;Chapter 1. INTRODUCTION;12
7;Chapter 2. THEORY;15
8;Chapter 3. GENERAL METHODS;26
8.1;Method I: "Kapillaranalyse";26
8.2;Method II: Descending Chromatography;26
8.3;Method III: Ascending Chromatography;29
8.4;Method IV: Ascending-Descending Chromatography;32
8.5;Method V: Filter Paper "Chromatopile";33
8.6;Method VI: "Reversed Phase" Paper Chromatography;35
8.7;Method VII: Circular Filter Paper Chromatography;35
8.8;Method VIII: Microbiological and Enzymatic Methods;36
8.9;Method IX: Paper Electrophoresis;36
9;Chapter 4. QUANTITATIVE METHODS;44
9.1;Method I: Visual Comparison;44
9.2;Method II: Elution;46
9.3;Method III: Area of Spot;49
9.4;Method IV: Total Color of Spot;51
9.5;Method V: Maximum Color Density;55
9.6;Method VI: Area X Density Method;60
9.7;Method VII: Retention Analysis;60
10;Chapter 5. AMINO ACIDS, AMINES AND PROTEINS;62
10.1;Section I: Amino Acids and Peptides;62
10.2;General Directions;62
10.3;Section II: Identification of Dinitrophenyl Amino Acids and Amino Alcohols;81
10.4;Section III: Identification of Amines;84
10.5;Section IV: Separation of Proteins;87
11;Chapter 6. CARBOHYDRATES;91
11.1;General Directions;91
11.2;Section I: Simple Sugars;91
11.3;Section II: Di- and Polysaccharides, Starch, and Cellulose;97
11.4;Section III: Substituted Sugars;98
11.5;Section IV: Miscellaneous Derived Sugars;103
12;Chapter 7. ALIPHATIC ACIDS AND STEROIDS;105
12.1;Section I: Aliphatic Acids General Directions;105
12.2;Section II: a-Keto Acids;111
12.3;Section III: Steroids;113
13;Chapter 8. PURINES, PYRIMIDINES, AND RELATED SUBSTANCES;127
13.1;General Directions;127
14;Chapter 9. PHENOLS, AROMATIC ACIDS, AND PORPHYRINS;136
14.1;Section I;136
14.2;Section II: Porphyrins;142
15;Chapter 10. MISCELLANEOUS ORGANIC SUBSTANCES;146
15.1;Experimental Details;146
16;Chapter 11. ANTIBIOTICS AND VITAMINS;157
16.1;Section I: Antibiotics;157
16.2;Section II: Vitamins;164
17;Chapter 12. INORGANIC SEPARATIONS;171
17.1;General Directions;172
17.2;Experimental Details;182
17.3;Comments;183
18;BIBLIOGRAPHY;184
19;AUTHOR INDEX;194
20;SUBJECT INDEX;200
