Bullock Techniques in Clinical Chemistry

A Handbook for Medical Laboratory Technicians
1. Auflage 2013
ISBN: 978-1-4832-2126-7
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 182 Seiten, Web PDF

Techniques in Clinical Chemistry: A Handbook for Medical Laboratory Technicians is a five-chapter supplementary handbook on the fundamentals of medical laboratory test and protocols. The opening chapter covers the fundamental aspects of medical laboratories, including accuracy measures, methods of analysis, buffers, indicators, and valency. These topics are followed by discussions on the procedures for preliminary preparation, primarily for sample preparation and specimen collection. Considerable chapters are devoted to the scrutinized analysis of specific specimen, such as blood, cerebrospinal fluid, feces, gastric sample, and urine. The final chapter discusses technical essentials of renal and hepatic function tests. This handbook is directed toward medical and laboratory technicians and clinicians.

Autoren/Hrsg.

Bullock, Frederick N.

Inhaltsverzeichnis

1;Front Cover;1
2;Techniques in clinical Chemistry: A Handbook for Medical Laboratory Technicians;4
3;Copyright Page;5
4;Table of Contents;8
5;Preface;7
6;CHAPTER I. FUNDAMENTALS;12
6.1;ACCURACY;12
6.2;VOLUMETRIC ANALYSIS;19
6.3;STANDARD SOLUTIONS;21
6.4;TYPES OF VOLUMETRIC ANALYSIS;22
6.5;IODOMETRIC STANDARDIZATION OF SOLUTIONS;25
6.6;HYDROGEN ION CONCENTRATION;28
6.7;BUFFERS;31
6.8;INDICATORS;31
6.9;VALENCY;35
7;CHAPTER II. PRELIMINARY PREPARATION;37
7.1;THE CLEANING OF GLASSWARE;37
7.2;DETERGENTS;38
7.3;THE COLLECTION OF PATHOLOGICAL SPECIMENS AND THEIR SUBMISSION TO THE LABORATORY;39
7.4;THE COLLECTION OF BLOOD SPECIMENS;40
7.5;THE COLLECTION OF URINE SPECIMENS;44
7.6;THE COLLECTION OF FÆCES;45
7.7;THE COLLECTION OF CEREBROSPINAL FLUID;46
7.8;THE COLLECTION OF GASTRIC CONTENTS;46
7.9;THE COLLECTION OF EXUDATES AND TRANSUDATES;47
7.10;THE PREPARATION OF THE PROTEIN-FREE FILTRATE;47
8;CHAPTER III. EXAMINATION OF BLOOD;50
8.1;ESTIMATION OF BLOOD-UREA;50
8.2;ESTIMATION OF NON-PROTEIN NITROGEN;56
8.3;ESTIMATION OF BLOOD URIC ACID;63
8.4;ESTIMATION OF BLOOD-GLUCOSE;67
8.5;ESTIMATION OF TOTAL BLOOD-CHOLESTEROL;72
8.6;ESTIMATION OF SERUM-CALCIUM;78
8.7;ESTIMATION OF PLASMA-CHLORIDES;81
8.8;ESTIMATION OF PLASMA-PROTEINS;84
8.9;ESTIMATION OF PLASMA-SODIUM;87
8.10;ESTIMATION OF SERUM-MAGNESIUM;90
8.11;ESTIMATION OF BLOOD-IRON;93
8.12;ESTIMATION OF SERUM INORGANIC PHOSPHORUS;96
8.13;ESTIMATION OF SERUM-POTASSIUM;98
8.14;ESTIMATION OF SERUM-BROMIDES;102
8.15;ESTIMATION OF SERUM-PHOSPHATASE;103
8.16;ESTIMATION OF SERUM ASCORBIC ACID (VITAMIN C);107
8.17;ESTIMATION OF THE CARBON-DIOXIDECOMBINING POWER OF PLASMA;110
8.18;DETECTION AND ESTIMATION OF SERUM-BILIRUBIN;113
9;CHAPTER IV. CEREBROSPINAL FLUID ANALYSIS;116
9.1;ESTIMATION OF CHLORIDES;116
9.2;ESTIMATION OF UREA AND GLUCOSE;118
9.3;ESTIMATION OF TOTAL PROTEIN;118
9.4;'GLOBULIN ' REACTIONS;120
10;CHAPTER V. ANALYSIS OF THE FÆCES;125
10.1;ESTIMATION OF FAT IN FÆCES;125
10.2;DETECTION OF OCCULT BLOOD IN FÆCES;130
10.3;BILIRUBIN;131
10.4;UROBILIN;131
11;CHAPTER VI. GASTRIC ANALYSIS;132
11.1;ESTIMATION OF GASTRIC CHLORIDES;132
11.2;DETECTION OF ORGANIC ACIDS;134
11.3;ESTIMATION OF FREE AND COMBINED HYDROCHLORIC ACID IN GASTRIC CONTENTS;135
11.4;THE FRACTIONAL TEST-MEAL;136
12;CHAPTER VII. EXAMINATION OF URINE;138
12.1;DETECTION AND ESTIMATION OF GLUCOSE;138
12.2;THE GLUCOSE-TOLERANCE TEST;140
12.3;DETECTION OF KETONE BODIES;141
12.4;ESTIMATION OF URINE CHLORIDES;143
12.5;ESTIMATION OF URINE UREA;144
12.6;ESTIMATION OF URINARY DIASTASE;147
12.7;ESTIMATION OF URINE ASCORBIC ACID;149
12.8;PROTEINURIA;151
12.9;INDICANURIA;155
12.10;BILIRUBIN AND UROBILIN;156
12.11;BLOOD—HÆMATURIA AND HÆMOGLOBINURIA;158
12.12;MELANOGENURIA;158
12.13;ALKAPTONURIA;158
12.14;PORPHYRINURIA;159
13;CHAPTER VIII. TEST OF RENAL AND HEPATIC FUNCTION;161
13.1;TESTS FOR RENAL EFFICIENCY;161
13.2;LIVER FUNCTION TESTS;164
14;APPENDIX;166
14.1;I. NORMAL BLOOD VALUES;166
14.2;2. ATOMIC WEIGHTS;167
14.3;3. CALIBRATION OF GLASSWARE WITH MERCURY;167
14.4;4. DICHROMATE CLEANING FLUID;168
14.5;5· PREPARATION OF STOP-COCK GREASE;168
14.6;6. NORMALITY OF CONCENTRATED ACIDS AND BASES;168
14.7;7. ACCURACY OF VOLUMETRIC FLASKS;169
14.8;8 . SPECIFICATIONS FOR GRADUATED PIPETTES;169
14.9;9. SPECIFICATIONS FOR ONE-MARK BULB PIPETTES;172
14.10;10. SPECIFICATIONS FOR BURETTES;173
15;INDEX;174
16;NOTES;183