Bures / Petran / Zachar Electrophysiological Methods in Biological Research

3. Auflage 2013
ISBN: 978-1-4832-6145-4
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 824 Seiten, Web PDF

Electrophysiological Methods in Biological Research, Third Revised Edition describes the principles and applications of significant electrophysiological methods as regards to transistorisation of electrophysiological apparatus and to the mathematical analysis of electrophysiological data. The book explains the aspects of physics and electronics that are important in electrophysiology, such as the basic principles of semiconductor function, electronic simulators, electrodes, and the processing of electrophysiological data. The text also cites several examples that measure the resulting membrane potential if one electrode is inside the cell while the other is in contact with the cell's surface. Other experiments show the electrophysiological techniques and the fundamentals of electrical activity in the peripheral excitable structures, and its association with physiological functions. In considering the problems of nerve and muscle physiology, the investigator should know the technique of recording the electrical signs of a nerve impulse. These signs, or action potentials, indicate the presence of a nerve impulse. The text also discusses the effects of barbiturates or ether anesthesia in EEG activity, as well as its dissociation after physostigmine and atropine have been administered. The book can prove useful for pharmacologists, microchemists, cellular biologists, and research workers and technologists dealing with neural mechanisms.

Autoren/Hrsg.

Bures, Jan

Petran, Mojmir

Zachar, Jozef

Inhaltsverzeichnis

1;Front Cover;1
2;Electrophysiological Methods in Biological Research;4
3;Copyright Page;5
4;Table of Contents;6
5;Foreword to the third edition;14
6;Preface;16
7;Introduction;18
8;CHAPTER I. Theoretical basis of electrophysiological phenomena;24
8.1;A. Some fundamentals of electrochemistry;24
8.2;B. The membrane theory of bioelectric phenomena;32
9;CHAPTER II. Electrophysiological apparatus and technique;41
9.1;A. Stimulation technique;42
9.2;B. Basic principles of valve and semiconductor function;59
9.3;C. Electronic stimulators;82
9.4;D. Recording technique;130
9.5;E. Location of faults and interferences;192
9.6;F. Electrodes;207
9.7;G. Processing of electrophysiological data;231
10;CHAPTER III. General electrophysiology of cells and tissues;272
10.1;A.. Electric potentials of cells;272
10.2;B. Electric potentials of tissues;284
10.3;C. Electric phenomena in plants;295
10.4;D. Electric polarity of the animal organism;301
11;CHAPTER IV. Electrophysiology of isolated excitable structures In vitro;305
11.1;A. Electric manifestations of a nerve impulse;305
11.2;B. Propagation of nervous impulses;327
11.3;C. The initiation of nerve impulses;343
11.4;D. Recovery processes following a nerve impulse;371
11.5;E. Electrophysiology of the isolated skeletal muscle;379
12;CHAPTER V. Electrophysiology of peripheral excitable structures In situ;388
12.1;a) Recording of an impulse in a volume conductor;388
12.2;b) Impulse activity of somatic and vegetative nerves;392
12.3;c) Impulse activity of muscle in situ. Electromyogram;397
12.4;d) Recording of nerve and muscle action potentials through the intact skin in man;401
13;CHAPTER VI. Electrophysiology of peripheral synoptic junctions;406
13.1;A. Neuromuscular transmission in skeletal muscle;406
13.2;B. Synaptic transmission in a sympathetic ganglion;428
13.3;C. Electrically transmitting junctions;437
14;CHAPTER VII. Electrophysiology of sensory receptors;446
14.1;A. Models of sensory organs;446
14.2;B. Impulse activity in sensory nerve fibres;450
14.3;C. Receptor potentials;454
15;CHAPTER VIII. Electrophysiology of the spinal cord;472
15.1;A. Extracellular recording;473
15.2;B. Intracellular recording;497
16;CHAPTER IX. Electrophysiology of the cerebral cortex;508
16.1;A. Electroencephalography and electrocorticography in general;508
16.2;B. Spontaneous EEG and ECoG in animals;513
16.3;C. Drug effects on EEG;519
16.4;D. Primary cortical responses. Localization of primary responses in rat and cat. Primary responses to repeated stimuli Secondary responses;526
16.5;E. Responses of the cerebral cortex to direct electrical stimulation;542
16.6;F. Mapping of nervous pathways in the central nervous system using neuronography;547
16.7;G. Depth recording in the cerebral cortex;554
16.8;H. Antidromic and orthodromic stimulation of pyramidal pathway;558
16.9;I. Steady potentials and impedance of the cerebral cortex;563
16.10;J. Spreading depression;569
16.11;K. Theoretical basis of recording electric potentials in a volume conductor;580
17;CHAPTER X. Electrophysiology of subcortlcal structures;587
17.1;A. Stereotaxic method;587
17.2;B. Primary responses in the subcortical centres of afferent systems;594
17.3;C. Electric activity of the cerebellum;600
17.4;D. Electric activity of the hippocampus;605
17.5;E. Nonspecific subcortical influences on the cerebral cortex;610
17.6;F. Unit activity of reticular neurons;616
17.7;G. Simple methods for evaluating unit activity;625
17.8;H. Application of the functional ablation technique in analysing the cortico-subcortical relationships;635
17.9;I. Electrophysiological signs of an epileptic seizure;646
18;Appendix I: Stereotaxic atlases for the cat, rabbit and rat;654
19;Appendix II: Mathematical terms;733
20;References;748
21;Author index;791
22;Subject index;802