Castillo / Echávarri / Vallés Doubled Haploid Methods in Cereals

Erscheinungsjahr 2026
ISBN: 978-1-0716-5245-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 348 Seiten

Reihe: Springer Protocols

This volume addresses the ongoing need for progress in cereal doubled haploid (DH) technology by presenting recent developments that contribute to advances in this field. It brings together a series of general chapters that explore key questions in cereal breeding and DH production, along with detailed and up-to-date protocols for generating DH lines for use in breeding programs. In addition, the book includes methodologies for morphological, biochemical, molecular, and cytogenetic characterization that support and complement these protocols. Written for the highly successful  series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough,  aims to present up-to-date, reproducible approaches to inspire and empower researchers and breeders to develop, refine, and implement DH systems across diverse cereal species.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Castillo, Ana María

Echávarri, Begoña

Vallés, María Pilar

Inhaltsverzeichnis

Current Challenges in Cereal Breeding.- Molecular Insights into Microspore Embryogenesis Transcriptomic Dynamics in Barley and Wheat.- Albinism in Cereal Androgenesis Shedding Light on Molecular Mechanisms and Possible Solutions.- Production of Wheat Doubled Haploids Through Intergeneric Hybridization with Maize.-Wheat Doubled Haploid Production Through Maize Crossing.- Bread Wheat (Triticum aestivum L.) Doubled Haploid Production by Interspecific Crosses with Imperata .- Hexaploid Wheat × Barley (Triticum aestivum × Hordeum vulgare) Intergeneric Hybrids: Production and Analysis.- Bread Wheat Anther Culture.- Bread Wheat Microspore Culture.- Durum Wheat Anther Culture: A Complete Protocol.- Barley Doubled Haploid Plant Production by Anther Culture.- Monitoring Barley Microspore Embryogenesis: In Vitro Culture and Immunolocalization Analyses.- Production of Homozygous Maize Lines using Maternal Haploid Inducer lines.- In Vitro Anther Culture of Japonica and Indica Rice.- Indica Rice Anther and Shed Microspore Culture.-  Isolated Microspore Cultures of Triticale (× Wittm).- Efficient Protocol for Doubled Haploid Production in Rye (Secale cereale L.).- Targeted Gene Editing in Wheat during Haploid Production via Wide Hybridization with Transgenic Maize Expressing Cas9 and Guide RNA.- In Vivo Rice Haploid-induction System by an Egg Cell-specific Peptidase Knockout.