Chen Thermomorphogenesis

Methods and Protocols
Erscheinungsjahr 2024
ISBN: 978-1-0716-3814-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 264 Seiten

Reihe: Methods in Molecular Biology

This detailed collection explores thermomorphogenesis, a discipline that unravels the intricacies of warm temperature responses in plants. How plants perceive, interpret, and respond to elevated temperatures has far-reaching implications for agriculture, ecology, and our fundamental understanding of plant development, and this book presents techniques to help researchers delve into this area of study. Beginning with a section on thermomorphogenesis responses, the volume continues with numerous chapters on temperature sensing and temperature signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Thermomorphogenesis: Methods and Protocols serves as an ideal guide to this vital subject for research laboratories, as well as for graduate and undergraduate laboratory courses in plant molecular biology.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Chen, Meng

Inhaltsverzeichnis

A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring.- Dissection of Daytime and Nighttime Thermoresponsive Hypocotyl Elongation in Arabidopsis.- Light-Dependent High Ambient Temperature-Induced Senescence Assay Using Whole Seedlings.- Assessing Temperature Responses in Roots.- A Luciferase Imaging-Based Assay for Studying Temperature Compensation of the Circadian Clock.- Investigating the Effects of Temperature on Pathogen Propagation in Arabidopsis.- Assessing the High Temperature Effects on Stomatal Production.- Characterizing the Synergistic Response Induced by Warm Temperatures and Shade.- Measurement of Phytochrome B Thermal Reversion Rates In Vivo.- Characterization of Thermoresponsive Photobody Dynamics.- Phase Separation of Phytochrome B in HEK293T Cells.- Isolation of Phytochrome B Photobodies.- Analysis of Phase Separation of EARLY FLOWERING 3.- In Vitro Determination of Temperature-Dependent DNA-Binding of the Evening Complex Using Electrophoretic Mobility Shift Assays.- Monitoring Real-Time Temperature Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and Circular Dichroism.- Characterization of PIF4 Phosphorylation by SPA1.- Chromatin Immunoprecipitation to Investigate H2A.Z Dynamics in Response to Environmental Changes.- Assessing the Function of CBF1 in Modulating the Interaction between Phytochrome B and PIF4.- GST Pull-Down Assay to Study PIF4 Binding In Vitro.- Investigating Circadian Gating of Temperature Responsive Genes.- RuBisCO Depletion and Subcellular Fractionation for Enhanced Florigen Detection in Arabidopsis.- Identifying Causal Genes for Thermo-Responsive Rosette Growth in Arabidopsis by Genome-Wide Association Study.- Mathematical Modeling of Photo- and Thermomorphogenesis in Plants.