Day / Davidson The Fluorescent Protein Revolution

Erscheinungsjahr 2012
ISBN: 978-1-4398-7509-4
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 348 Seiten

Reihe: Series in Cellular and Clinical Imaging

This reference discusses major advances in the development of novel imaging techniques that exploit new probes. It describes the photophysical properties of new fluorescent probes, particularly the important red fluorescent probes from corals. The book gives a detailed account of state-of-the-art imaging methods that exploit the characteristics of new probes. It also illustrates the applications of fluorescent probes in a variety of optical techniques, including superresolution microscopy. Many figures throughout the text further clarify concepts.

Zielgruppe

Biomedical researchers, professionals in the optical sciences, and advanced undergraduate and graduate students in biomedical engineering.

Autoren/Hrsg.

Day, Richard N.

Davidson, Michael W.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Bildgebende Verfahren, Nuklearmedizin, Strahlentherapie Radiologie, Bildgebende Verfahren
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe
• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

HISTORY AND PERSPECTIVES
Introduction to Fluorescent Proteins; Ward
Development of Aequorea victoria Fluorescent Proteins; Miyawaki
The Quest for New Fluorescent Proteins from Corals; Matz, Lukyanov,and Wiednmann

PHOTOPHYSICAL PROPERTIES OF FLUORESCENT PROTEINS
Lessons Learned from the Structure of Aequorea Victoria Fluorescent Proteins; Remington
Development of Optimized Fluorescent Proteins; Waldo
Development of New Colors from Coral Fluorescent Proteins; Campbell
The Search for the Grail: Optimal Red Fluorescent Proteins; Davidson, Verkhusha, and Wiedenmann
Optical Highlighters; Lukyanov, Chudakov, and Davidson
Far Red and Near Infared Fluorescent Proteins; Paul Steinbach, Shu, and Lin

APPLICATIONS
Genetically Encoded Fluorescent Proteins and FRAP; McNally
Optical Highlighters; Lippincott-Schwartz and Patterson
Biosensor Probes; Zhang
Probes for FRET and FLIM; Day
Superresolution Techniques; Davidson
Whole Animal Imaging with Red, Near-Infrared Probes; Tsien
Future Prospects; Davidson and Day