de Folter Plant MicroRNAs

Methods and Protocols
1. Auflage 2019
ISBN: 978-1-4939-9042-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 363 Seiten

Reihe: Springer Protocols

This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

de Folter, Stefan

Inhaltsverzeichnis

An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants.- The Plant MicroRNA Pathway: The Production and Action Stages.- Plant miRNA Conservation and Evolution.- Bioinformatic Analysis of Small RNA Sequencing Libraries.- An Overview of miRNA and miRNA Target Analysis Tools.- miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences.- Prediction of Plant miRNA Targets.- Isolation and Detection Methods of Plant miRNAs.- Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants.- miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses.- Detection of miRNAs by Tissue Printing and Dot Blot Hybridization.- In Situ Localization of Small RNAs in Plants.- Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs.- A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy.- Degradome Sequencing in Plants.-Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System.- The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis.- Mimicry Technology: A Versatile Tool for Small RNA Suppression.- Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants.- Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches.- Analysis of Methylation Status of Plant MicroRNAs.- Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1.- The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs.- Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae.- Detection of Histone Modifications Associated with miRNAs.