Ferrer-Montiel / Hucho TRP Channels

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-9446-5
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1987, 238 Seiten, eBook

Reihe: Methods in Molecular Biology

This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies. Articles cover a wide range of techniques, from structure to function, concluding with how to best to evaluate some of the in vivo function of TRP channels, particularly in regard to their involvement in hyperalgesia and allodynia. Also, the collection describes important and inspiring available tools that are currently being used to investigate the activity of these channels in vitro and in vivo in terms of their underlying protein structure, segueing into dealing with the generation of pharmacological tools to explore their in vivo activity and their involvement in cellular signaling pathways, which may be developed into drugs to treat diseases caused by channel dysfunction or hyperactivity. Written for the highly successful Methods in Molecular Biology series, chapters include the necessary detail and implementation advice to ensure successful results in the lab.  Authoritative and practical, TRP Channels: Methods and Protocols is a useful set of methodologies for scientists seeking to better understand this vital family of channels.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Ferrer-Montiel, Antonio

Hucho, Tim

Inhaltsverzeichnis

Molecular Evolution Bioinformatics towards Structural Biology of TRPV1-4 Channels.- Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6.- Cryo-Electron Microscopy of TRP Channels.- Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation.- Combining Structural Data with Computational Methodologies to Investigate Structure-Function Relationships in TRP Channels.- Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique.- Fluorescence-Based Functional Assays for Ca 2+ Permeable ThermoTRP Channels.- High Content Imaging of Immunofluorescently-Labeled TRPV1-Positive Sensory Neurons.- Patch-Clamp Combined with Fast Temperature Jumps to Study Thermal TRP Channels.- TRP Channels Reconstitution in Lipid Bilayers.- Methods for Investigating TRP Channel Gating.- In Silico Approaches for TRP Channel Modulation.- Drug Discovery for Soft Drugs on TRPV1 and TRPM8 Channels Using the Passerini Reaction.- In Vivo Methods to Study ThermoTRP Channels in Rodents.