Friedrich / Gilbert Cell Viability Assays

Methods and Protocols
2. Auflage 2023
ISBN: 978-1-0716-3052-5
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 502 Seiten

Reihe: Springer Protocols

This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environments.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Friedrich, Oliver

Gilbert, Daniel F.

Inhaltsverzeichnis

Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness.- The MTT Assay: A Method for Error Minimization and Interpretation in Measuring Cytotoxicity and Estimating Cell Viability.- Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions.- Network Reconstruction as a Novel High-Level Marker of Functional Neuronal Viability.- Assaying Mitochondrial Function by Multi-Parametric Flow Cytometry.- High-Efficiency Single Cell Electrical Impedance Spectroscopy.- Cell Viability Multiplexing: Quantification of Cellular Viability by Barcode Flow Cytometry and Computational Analysis.- Average Rheological Quantities of Cells in Monolayers.- Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arrays.- Quantitative Live-Cell Ca Imaging During Isotropic Cell Stretch.- Assessment of Cell Viability in Electrically Excitable Muscle Cells through Intact Twitch Stimulation.-Evaluating Cellular Viability by iTRAQ Proteomic Profiling.- Quantifying pH in Malaria Using pHluorin and Flow Cytometry.- Cell Membrane State, Permeability, and Elasticity Assessment for Single Cells and Cell Ensembles.- Neutral Red Uptake Assay to Assess Cytotoxicity In Vitro.- Digital Holographic Microscopy to Assess Cell Behavior.- Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells.- Optimization of Cell Viability Assays for Drug Sensitivity Screens.- Cellasys #8: A Microphysiometric Assay for Real-Time Cell Analysis within 24 Hours.- Live Cell Adhesion, Migration, and Invasion Assays.- Cell Viability and Immunogenic Function of T Cells Loaded with Nanoparticles for Spatial Guidance in Magnetic Fields.- Transwell In Vitro Cell Migration and Invasion Assays.- Live Cell Calcium Imaging of Non-Adherent Cells.- FUCCI Reporter Gene-Based Cell Cycle Analysis.- Cell Viability Assays for 3D Cellular Constructs.- Toxicological Analysis by Assessment of Vascularization and Cell Viability Using the Chicken's Chorioallantoic Membrane (CAM Assay).- Assessment of Tissue Viability by Functional Imaging of Membrane Potential.- Multiplexed Viability Assays for High-Throughput Screening of Spheroids of Multiple Sizes.- Tracking Gut Motility in Organ and Cultures.- Optimized Method of 3D Scaffold Seeding, Cell Cultivation, and Monitoring Cell Status for Bone Tissue Engineering.