Gautier / Hinner Site-Specific Protein Labeling

Methods and Protocols
2015
ISBN: 978-1-4939-2272-7
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1266, 267 Seiten, eBook

Reihe: Methods in Molecular Biology

This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Gautier, Arnaud

Hinner, Marlon J.

Inhaltsverzeichnis

1. How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine TagB. Albert Griffin, Stephen R. Adams, and Roger Y. Tsien 2. Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug DiscoveryLukas Leder 3. Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins Nicole J. Yang and Marlon J. Hinner 4. Considerations and Protocols for the Synthesis of Custom Protein Labeling ProbesIvan R. Corrêa Jr. 5. 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine ResiduesLina Cui and Jianghong Rao 6. Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion ChannelsThomas K. Berger and Ehud Y. Isacoff 7. Fluorescent Labeling Of SNAP-Tagged Proteins In CellsGražvydas Lukinavicius, Luc Reymond, and Kai Johnsson 8. HaloTag Technology For Specific And Covalent Labeling Of Fusion ProteinsHélène A. Benink and Marjeta Urh 9. Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-SplicingJulian Matern, Anne-Lena Bachmann, Ilka V. Thiel, Gerrit Volkmann, Alexandra Wasmuth, Jens Binschik and Henning D. Mootz 10. Chemical-tag Labeling Of Proteins Using Fully Recombinant Split InteinsAnne-Lena Bachmann, Julian Matern, Vivien Schütz, and Henning D. Mootz 11. Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein LabelingBo Zhao, Keya Zhang, Karan Bhuripanyo, Yiyang Wang, Han Zhou, Mengnan Zhang, and Jun Yin 12. Site-Specific Biotinylation Of Purified Proteins Using BirAMichael Fairhead and Mark Howarth 13. Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular BiologistMaximilian Wei-Lin Popp 14. BONCAT – Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized ProteomesPeter Landgraf, Elmer R. Antileo, Erin M. Schuman, and Daniela C. Dieterich 15. Genetic Encoding Of Unnatural Amino Acids For Labeling ProteinsKathrin Lang, Lloyd Davis,  and Jason W. Chin 16. Labeling Proteins By Affinity-Guided DMAP ChemistryTomonori Tamura and Itaru Hamachi 17. Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In VivoShinya Tsukiji and Itaru Hamachi