Giedt / Tootle Drosophila Oogenesis

Methods and Protocols
Erscheinungsjahr 2023
ISBN: 978-1-0716-2970-3
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 447 Seiten

Reihe: Methods in Molecular Biology

This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing chapters exploring how Drosophila oogenesis can be used in the classroom and in outreach programs to increase interest in biomedical research, STEM education, and STEM careers. Written for the highly successful  Methods in Molecular Biology  series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical,  Drosophila Oogenesis: Methods and Protocols  serves as an ideal guide for both new and expert researchers working with this robust and versatile model organism.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Giedt, Michelle S.

Tootle, Tina L.

Inhaltsverzeichnis

The Vast Utility of Drosophila Oogenesis.- Visualization and Quantification of Drosophila Larval Ovaries.- Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries.- Utilizing the FLP-Out System for Clonal RNAi Analysis in the Adult Drosophila Ovary.- Analysis of Physiological Control of Adult Drosophila Oogenesis by Inter-Organ Communication.- Immunohistochemical Analysis of Nuclear Lamina Structures in the Drosophila Ovary Using CRISPR-Tagged Genes.- Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells.- Tracking Follicle Cell Development.- Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells.- Quantitative Image Analysis of Dynamic Cell Behaviors during Border Cell Migration.- Live Imaging of Nurse Cell Behavior in Late Stages of Drosophila Oogenesis.- Visualizing Lipid Droplets in Drosophila Oogenesis.- Assessing Ovulation in Drosophila melanogaster .- A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers during Drug Treatments.- Detection and Assessment of Wolbachiapipientis Infection.- Measuring Transposable Element Activity in Adult Drosophila Ovaries.- Preparation of Drosophila Ovarioles for Single-Cell RNA Sequencing.- Chromatin Immunoprecipitation Experiments from Drosophila Ovaries.- Detection of Actin in Nuclear Protein Fraction Isolated from Adult Drosophila Ovary.- STAMP: Spatio-Temporal Association Mapping of Proteins.- Using Drosophila Oogenesis in the Classroom to Increase Student Participation in Biomedical Research.- Gaining Wings to FLY: Using Drosophila Oogenesis as an Entry Point for Citizen Scientists in Laboratory Research.