Gillespie Antibiotic Resistance Methods and Protocols

Erscheinungsjahr 2008
ISBN: 978-1-59259-077-3
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 48, 287 Seiten, eBook

Reihe: Methods in Molecular Medicine

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Gillespie, Stephen H.

Inhaltsverzeichnis

Diagnosis of Resistance.- Multiplex Polymerase Chain Reaction Detection of vanA, vanB, vanC-1, and vanC-2/3 Genes in Enterococci.- Drug Susceptibility of Mycobacterium tuberculosisThrough the Mycolic Acid Index.- Micro-Well Phage Replication Assay for Screening Mycobacteria for Resistance to Rifampin and Streptomycin.- Application of SSCP to Identification of Resistance Mutations.- Quantitative, Single-Tube, Nested, PCR (QSTN-PCR) for Determining the Antibiotic Susceptibility of Mycobacterium tuberculosis.- Rapid Rifamycin Susceptibility Testing of Small-Volume Mycrobacterium tuberculosis Cultures by Detection of Precursor rRNA.- Detection of Penicillin Resistance in Streptococcus pneumoniae by a Seminested PCR Strategy.- Diagnosis of Penicillin Resistance by PCR-RFLP.- Detection Methods of Glycopeptide-Resistant Staphylococcus aureus I.- Detection Methods for Glycopeptide-Resistant Staphylococcus aureus II.- Multiplex PCR for the Rapid Simultaneous Speciation and Detection of Methicillin-Resistance and Genes Encoding Toxin Production in Staphylococcus aureus.- Chromogenic Detection of Aminoglycoside Phosphotransferases.- Monitoring Response to Treatment.- Quantification of M. tuberculosis DNA in Sputum During the Treatment of Pulmonary Tuberculosis.- Isolation of M. tuberculosis RNA from Sputum.- Detection of Viable Mycobacterium tuberculosis by Reverse Transcriptase-Strand Displacement Amplification of mRNA.- Molecular Epidemiology.- BOX PCR Fingerprinting for Molecular Typing of Streptococcus pneumoniae.- Restriction Fragment End Labeling Analysis: High-Resolution Genomic Typing of Streptococcus pneumoniae Isolates.- Pulse-field Gel Electrophoresis for Epidemiological Studies of Streptococcus pneumoniae.- Outer Membrane Profiles of Clonally Related Klebsiella pneumoniae.- Atomic Force Microscopy.- Biology of Resistance.- Assessing the Activity of Bacterial Multidrug Efflux Pumps.- The Use of a Continuous Culture System to Study the Antimicrobial Susceptibility of Bacteria in Biofilm.- Estimation of Mutation Rates in Antibiotic Research.- In Vitro Assessment of the Fitness of Resistant M. tuberculosis Bacteria by Competition Assay.- Purification of DNA Topoisomerases and Inhibition by Fluoroquinolones.- Site-Directed Mutagenesis to Determine Structure Function Relationships in Streptococcus pneumoniae Penicillin-Binding Protein Genes.- Detection of Low Affinity Penicillin-Binding Protein Variants in Streptococcus pneumoniae.- Transmission of Resistance.- Mobilization of Transposons.