Gillette / Conney / Cosmides Microsomes and Drug Oxidations

1. Auflage 2013
ISBN: 978-1-4832-6633-6
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 562 Seiten, Web PDF

Microsomes and Drug Oxidations documents the proceedings of symposium on microsomes and drug oxidations held at Bethesda, Maryland, 16-17 February 1968. The theme of the symposium centered on the morphology of the liver endoplasmic reticulum, the localization of enzyme systems, the mechanisms for regulating enzyme activity, and the mechanisms of enzyme action. The results of this symposium presented in this book illustrate the complexity of the enzyme systems under investigation and the multiplicity of the variables altering their function. It is hoped the information presented will serve as a stimulus to other investigators in the elucidation of these problems. The book contains 27 papers organized into four parts. The papers in Part I examine the morphological and biochemical characteristics of microsomes. Part II presents studies on electron transfer components. Part III examines alterations of microsomal enzymes while Part IV focuses on drugs and protein synthesis.

Autoren/Hrsg.

Gillette, James R.

Conney, Allan H.

Cosmides, George J.

Inhaltsverzeichnis

1;Front Cover;1
2;Microsomes and Drug Oxidations;4
3;Copyright Page;5
4;Table of Contents;12
5;Participants;6
6;Preface;10
7;PART I: MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF MICROSOMES;16
7.1;CHAPTER 1. MICROSOMES, ENDOPLASMIC RETICULUM AND INTERACTIONS OF CYTOPLASMIC MEMBRANES;18
7.1.1;Introduction;18
7.1.2;Properties of Lipid and Biological Membranes;23
7.1.3;Endoplasmic Reticulum;25
7.1.4;Peroxisomes and Smooth E.R.;29
7.1.5;Synthesis and Transport of Lipoprotein Granules;31
7.1.6;Smooth Endoplasmic Reticulum and Golgi Apparatus;33
7.1.7;References;35
7.2;CHAPTER 2. SUBFRACTIONATION OF RAT LIVER MICROSOMES;56
7.2.1;Abstract;56
7.2.2;Introduction;57
7.2.3;Discussion;66
7.2.4;Acknowledgement;70
7.2.5;References;70
7.3;CHAPTER 3. A POSSIBLE ROLE OF THE ENDOPLASMIC RETICULUM IN THE REGULATION OF GENETIC EXPRESSION THE MEMBRON CONCEPT;74
7.3.1;References;92
7.4;CHAPTER 4. THE METABOLISM OF DRUGS BY SUBFRACTIONS OF HEPATIC MICROSOMES: THE CASE FOR MICROSOMAL HETEROGENEITY;96
7.4.1;Conclusion;103
7.4.2;References;105
8;PART II: ELECTRON TRANSFER COMPONENTS;108
8.1;CHAPTER 5. ORGANIZATION OF THE MICROSOMAL ELECTRON TRANSPORT SYSTEM;110
8.1.1;Discussion;118
8.1.2;References;119
8.1.3;Discussion;120
8.2;CHAPTER 6. CHARACTERIZATION OF TWO HEMOPROTEINS OF LIVER MICROSOMES;126
8.2.1;Spectral Properties of P-450;128
8.2.2;Concluding Remarks;140
8.2.3;Acknowledgment;141
8.2.4;References;141
8.2.5;Discussion;144
8.3;CHAPTER 7. CYTOCHROME P-450 REDUCTION IN LIVER MICROSOMES AND ITS RELATIONSHIP TO DRUG METABOLISM;148
8.3.1;Reduction of azo- and nitro- and tetrazolium compounds;149
8.3.2;Distribution of NADPH-dependent enzyme activities in microsomal subfractions;154
8.3.3;Role of Various Components of the Electron Transfer Systemin Species Variation in Hepatic Microsomal Drug Metabolism;156
8.3.4;Effect of Drug Substrates on Hepatic Microsomal Cytochrome P-450 Reduction by NADPH
;157
8.3.5;References;163
8.3.6;Discussion;164
8.4;CHAPTER 8. FATTY ACID . - OXIDATION IN A SOLUBLE MICROSOMAL ENZYME SYSTEM CONTAINING P-450;166
8.4.1;Hemoprotein P-450;167
8.4.2;References;173
8.4.3;Discussion;175
8.5;CHAPTER 9. ENZYMATIC . -OXIDATION: STOICHIOMETRY OF THE REACTION CATALYZED BY THE INDUCIBLE ENZYME SYSTEM OF PSEUDOMONAS OLEOVORANS;182
8.5.1;References;186
8.6;CHAPTER 10. THE PROPERTIES OF A PURIFIED HEPATIC MICROSOMAL MIXED FUNCTION AMINE OXIDASE;188
8.6.1;Introduction;189
8.6.2;Experimental;189
8.6.3;Discussion;199
8.6.4;References;201
8.7;CHAPTER 11. A COMPARISON OF NADPH DEPENDENT REDUCTIVE CLEAVAGE AND N-DEMETHYLATION OF AMINOAZOBENZENE, MONOMETHYLAMINOAZO-BENZENE AND DIMETHYLAMINOAZOBENZENE BY CELL FREE PREPARATIONS FROM RAT LIVER;204
8.7.1;Results;207
8.7.2;Discussion;209
8.7.3;References;212
8.8;CHAPTER 12. OXYGEN REACTIONS IN MODEL SYSTEMS;214
8.8.1;References;231
8.9;CHAPTER 13. SIGNIFICANCE OF THE NIH SHIFT WITH RESPECT TO LIVER MICROSOME HYDROXYLATIONS;240
8.9.1;References;244
8.9.2;Discussion;246
8.10;CHAPTER 14. THE MICROSOMAL HYDROXYLATION OF ETHYLBENZENE STEREOCHEMICAL, INDUCTION AND ISOTOPIC STUDIES;254
8.10.1;Discussion;260
8.10.2;Acknowledgements;261
8.10.3;References;262
9;PART III: ALTERATIONS OF MICROSOMAL ENZYMES;264
9.1;CHAPTER 15. OVERALL BIOCHEMICAL EFFECTS OF DRUG INDUCTION ON LIVER

MICROSOMES;266
9.1.1;Acknowledgment;282
9.1.2;References;282
9.1.3;Discussion;285
9.2;CHAPTER 16. SPECIFICITY IN THE REGULATION OF THE 6ß- , 7a AND 16a-HYDROXYLATION OF TESTOSTERONE BY RAT LIVER MICROSOMES;294
9.2.1;Results;294
9.2.2;Summary;309
9.2.3;Acknowledgments;310
9.2.4;References;310
9.2.5;Discussion;311
9.3;CHAPTER 17. FORMATION OF A NEW P-450 HEMOPROTEIN AFTER TREATMENT OF RATS WITH POLYCYCLIC HYDROCARBONS;318
9.3.1;SKF 525-A Studies;320
9.3.2;Two-Substrate Kinetic Studies;321
9.3.3;Spectral Studies
;322
9.3.4;Binding Studies;326
9.3.5;Thioacetamide Studies;332
9.3.6;Summary;339
9.3.7;Acknowledgement;340
9.3.8;References;341
9.3.9;Discussion;342
9.4;CHAPTER 18. SPECTROPHOTOMETRIC STUDIES OF CYTOCHROME P-450 OF LIVER MICROSOMES AFTER INDUCTION WITH PHENOBARBITAL AND 3-METHYLCHOLANTHRENE
;346
9.4.1;References;358
9.4.2;Discussion;359
9.5;CHAPTER 19. THE EFFECTS OF 3-METHYLCHOLANTHRENE AND PHENOBARBITAL ON LIVER MICROSOMAL HEMOPROTEINS AND ON THE HYDROXYLATION OF BENZPYRENE
;364
9.5.1;Discussion;374
9.5.2;References;377
9.5.3;Discussion;379
9.6;CHAPTER 20. SPECTRAL INVESTIGATIONS ON CYTOCHROME-P-450;386
9.6.1;References;400
9.6.2;Discussion;401
10;PART IV: DRUGS AND PROTEIN SYNTHESIS;402
10.1;CHAPTER 21. DRUGS AND MICROSOMAL ENZYME FORMATION IN VIVO AND IN MAMMALIAN CELL CULTURE;404
10.1.1;References;436
10.1.2;Discussion;441
10.2;CHAPTER 22. ACTIVATION OF CHROMATIN BY 3-METHYLCHOLANTHRENE;446
10.2.1;Introduction;446
10.2.2;Discussion;459
10.2.3;Acknowledgements;461
10.2.4;References;461
10.2.5;Discussion;464
10.3;CHAPTER 23. INDUCTION, STABILIZATION AND TURNOVER OF ENDOPLASMIC RETICULUM PROTEINS;468
10.3.1;Summary;468
10.3.2;References;484
10.3.3;Discussion;486
10.4;CHAPTER 24. EFFECT OF PHENOBARBITAL ON THE TURNOVER OF MICROSOMAL ENZYMES;490
10.4.1;Methods;491
10.4.2;Results and Discussions;492
10.4.3;References;506
10.4.4;Discussion;507
10.5;CHAPTER 25. THE ROLE OF HEME IN THE SYNTHESIS AND REPRESSION OF MICROSOMAL PROTEIN;510
10.5.1;Methods;511
10.5.2;Results and Discussion;512
10.5.3;Acknowledgement;523
10.5.4;References;524
10.5.5;Discussion;527
10.6;CHAPTER 26. INSECT MICROSOMES AND INSECTICIDE CHEMICAL OXIDATIONS;532
10.6.1;Introduction;532
10.6.2;Resistance;539
10.6.3;Summary;542
10.6.4;Acknowledgement;542
10.6.5;References;542
10.6.6;Discussion;545
10.7;CHAPTER 27. STIMULATORY EFFECT OF ACETONE ON THE ACTIVITY OF MICROSOMAL ANILINE PARA-HYDROXYLASE;548
10.7.1;Summary;548
10.7.2;Introduction;548
10.7.3;Materials and Methods;549
10.7.4;Results;549
10.7.5;Discussion;553
10.7.6;References;554
11;SUBJECT INDEX;556