Givan Flow Cytometry

First Principles
2. Auflage 2013
ISBN: 978-1-118-68839-7
Verlag: John Wiley & Sons
Format: EPUB
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 304 Seiten, E-Book

Flow cytometry continually amazes scientists with itsever-expanding utility. Advances in flow cytometry have opened newdirections in theoretical science, clinical diagnosis, and medicalpractice. The new edition of Flow Cytometry: FirstPrinciples provides a thorough update of this now classic text,reflecting innovations in the field while outlining the fundamentalelements of instrumentation, sample preparation, and data analysis.
Flow Cytometry: First Principles, Second Edition explainsthe basic principles of flow cytometry, surveying its primaryscientific and clinical applications and highlightingstate-of-the-art techniques at the frontiers of research. Thisedition contains extensive revisions of all chapters, including newdiscussions on fluorochrome and laser options for multicoloranalysis, an additionalsection on apoptosis in the chapter on DNA,and new chapters onintracellular protein staining and cell sorting,including high-speed sorting and alternative sorting methods, aswell as traditional technology. This essential resource:
* Assumes no prior knowledge of flow cytometry
* Progresses with an informal, engaging lecture style fromsimpleto more complex concepts
* Offers a clear introduction to new vocabulary, principles ofinstrumentation, and strategies for data analysis
* Emphasizes the theory relevant to all flow cytometry, withexamples from a variety of clinical and scientific fields
Flow Cytometry: First Principles, Second Edition providesscientists, clinicians, technologists, and students with theknowledge necessary for beginning the practice of flow cytometryand for understanding related literature.

Autoren/Hrsg.

Givan, Alice Longobardi

Inhaltsverzeichnis

Preface.
Acknowledgments (First Edition).
Acknowledgments (Second Edition).
The Past as Prologue.
Setting the Scene.
Instrumentation: Into the Black Box.
Information: Harnessing the Data.
Seeing the Light: Lasers, Fluorochromes, and Filters.
Cells from Without: Leukocytes, Surface Proteins, and the Strategyof Gating.
Cells from Within: Intracellular Proteins.
Cells from Within: DNA in Life and Death.
The Sorting of Cells.
Disease and Diagnosis: The Clinical Laboratory.
Out of the Mainstream: Research Frontiers.
Flowing On: The Future.
General References.
Glossary.
Figure Credits.
Index.