Gorgoulis / Cavinato / Evangelou Oncogene-Induced Senescence

Methods and Protocols
Erscheinungsjahr 2025
ISBN: 978-1-0716-4426-3
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 363 Seiten

Reihe: Springer Protocols

This volume explores a collection of the most recent experimental approaches for the detection, modelling, characterization, and in-depth computational analysis of the biological phenomenon known as Oncogene-Induced Senescence (OIS). The chapters in this book cover topics such as protocols to study OIS in two-dimensional, three-dimensional, and in vivo (animal and human) settings; analyzing OIS using high-throughput bioinformatics; and discriminate OIS from aging. Published in the highly successful  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting to avoid known pitfalls.

Comprehensive and authoritative,  is a valuable resource for basic researchers and clinicians in the oncology field, and a great guide for computational scientists.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Gorgoulis, Vassilis G.

Cavinato, Maria

Evangelou, Konstantinos

Inhaltsverzeichnis

In Situ and In Silico Methods for Senescence Identification in Human Liver Diseases.- Current Methodologies to Assess Cellular Senescence in Cancer.- Selective Sorting of Senescent Cell Subpopulations Compatible with Downstream Genomics Pipelines.- Detection and Characterization of Senescent Cells with Flow Cytometry.-    Detecting Senescence in T Cells by Flow Cytometry Using the SA-ß-galactosidase Assay.- The DNA Damage Response as an Auxiliary Indicator of Senescence in Cancer: A User-Friendly Toolkit of Markers and Detection Methods.- Precise Mapping of Physiological DSBs Using In-Suspension Break Labelling In Situ and Sequencing (sBLISS).- Detecting Telomeric DNA Damage by Immuno-Telo FISH.- Combined In Situ Transcriptomic and Immunofluorescence to Assess the Effects on DNA Damage and Innate Immune Responses Induced by Bacterial Genotoxin in In Vivo Models.- Two-Replication Round Telomere Strand-Specific Chromosome Orientation Fluorescent In Situ Hybridization.- Identification of Oncogene-Induced Senescence-Associated MicroRNAs.- Global Proteomic Profiling to Unravel Lipofuscin’s Protein Repertoire.- Electron Microscopy to Visualize and Quantify Mitochondrial Structure and Organellar Interactions in Cultured Cells during Senescence.- Isolation and Characterization of EVs Containing Mitochondria from OIS Cells.- Isolation and Characterization of Endothelial Colony Forming Cells (ECFC): Studying Endothelial Senescence for Translational Studies and for Personalized Medicine.-  In Vitro Modeling of Vascular Senescence.- Studying Cellular Senescence Using the Model-Organism as a Screening Platform for Anti-Aging Compounds.- Natural Product-Derived Senotherapeutics: Extraction and Biological Evaluation Techniques.