Greaves / Roboz Mass Spectrometry for the Novice

1. Auflage 2012
ISBN: 978-1-4200-9420-6
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 309 Seiten

Designed specifically for novice users of mass spectronomy, an audience that is not currently well-addressed by existing texts, this book covers the basic concepts, instruments, and techniques with cases illustrating its application in problem solving. Other books in this field focus too narrowly on instrumentation without discussing practical application, or present the information in dense discussions and tables, making the information hard to find. This book does neither, it provides practical advice and key background knowledge in a format that is easy to use.

Autoren/Hrsg.

Greaves, John

Roboz, John

Fachgebiete

• Naturwissenschaften Chemie Analytische Chemie Massenspektrometrie, Spektroskopie, Spektrochemie

Inhaltsverzeichnis

Chapter 1 – An Overview
A brief history
Definitions concerning instruments, mass, m/z, and ions
Components of instrument and their functions
Definitions concerning instrument performance
Definitions concerning applications
Information from mass spectra
Diversity and scope of applications

Chapter 2 – Instrumentation
Sample introduction
Ion sources and methods of ionization
Mass analyzers
Ion current detectors

Vacuum systems
Data systems

Chapter 3 – Methodologies and Strategies
Measures of instrument performance
Interpretation of mass spectra
Analytical techniques and strategies
Omics
Biopolymers
Imaging mass spectrometry
Bioinformatics
Buying a mass spectrometer

Chapter 4 – Examples from Representative Publications
An open access mass spectrometry facility

Environmental: organochlorines in fish
Environmental: pharmaceuticals in surface and wastewaters
Pharmacology: Lipitor metabolism
New techniques: paper spray of pharmaceuticals
Petroleomics: crude oil characterization

Metabolomics: disease markers for a tropical disease
Metabolomics: chemical defense
Lipidomics: coronary artery disease
Proteomics: protein identification in a painting
Proteomics: protein identification and metastasis
Proteomics: noncovalent interactions
Tissue imaging: lipids in mouse tissues

Chapter 5 – The Absolute Essentials

Chapter 6 – Resources
Books, journals, review articles, classical publications

Major instrument manufacturers
Mass spectrometry societies, blogs, discussion groups
The mass spectrometrist and the Internet