Guilbault / Belcher / Freiser Enzymatic Methods of Analysis

International Series of Monographs in Analytical Chemistry
1. Auflage 2013
ISBN: 978-1-4831-5054-3
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 368 Seiten, Web PDF

Enzymatic Methods of Analysis attempts to cover all the aspects of modern enzymatic analysis. Enzymes possess a great potential usefulness in analytical chemistry. The specificity of enzymes can solve the primary problem of most analytical chemists-the analysis of one substance in the presence of many similar compounds that interfere in the analysis. The book begins with two introductory chapters devoted to general considerations of enzymes as reagents and methods of analysis of enzymatic reactions. The next four chapters deal with methods for the assay of specific enzymes, and substrate, activator, and inhibitor analysis using enzymes. In the last two chapters the immobilization of enzymes and the automation of enzymatic reactions are discussed. In addition, a listing of all commercially available enzymes is given in an appendix. It is hoped that the information presented will prove interesting and stimulating to all individuals engaged in research and development.

Autoren/Hrsg.

Guilbault, George G.

Belcher, R.

Freiser, H.

Inhaltsverzeichnis

1;Front Cover;1
2;Enzymatic Methods of Analysis;3
3;Copyright Page;4
4;Table of Contents;5
5;PREFACE;13
6;Dedication;17
7;CHAPTER 1. GENERAL CONSIDERATIONS;19
7.1;A. PRINCIPLES OF ENZYMATIC ANALYSIS;19
7.2;B. PROPERTIES OF ENZYMES;20
7.3;C. FACTORS INFLUENCING THE ENZYME REACTION RATE;24
7.4;D. DETERMINATION OF CONCENTRATIONS;35
7.5;E. HANDLING BIOCHEMICAL REAGENTS;38
7.6;F. SOURCES OF REAGENTS;40
7.7;G. ENZYME ACTIVITY;40
7.8;REFERENCES;41
8;CHAPTER 2. METHODS OF ASSAY;43
8.1;A. CHEMICAL METHODS;43
8.2;B. INSTRUMENTAL METHODS;43
8.3;REFERENCES;58
9;CHAPTER 3. DETERMINATION OF ENZYMES;61
9.1;A. GENERAL;61
9.2;B. HYDROLYTIC ENZYMES;61
9.3;C. OXIDATIVE ENZYMES;95
9.4;D. DEHYDROGENASE ENZYMES;110
9.5;E. KINASES AND TRANSAMINASES;119
9.6;F. OTHER ENZYMES;122
10;CHAPTER 4. DETERMINATION OF SUBSTRATES;132
10.1;A. GENERAL;132
10.2;B. CARBOHYDRATES;132
10.3;C. AMINES;145
10.4;D. AMINO ACIDS;149
10.5;E. ORGANIC ACIDS;159
10.6;REFERENCES;186
11;CHAPTER 5. DETERMINATION OF ACTIVATORS AND COENZYMES;194
11.1;A. GENERAL;194
11.2;B. DETERMINATION OF INORGANIC SUBSTANCES;194
11.3;C. DETERMINATION OF COENZYMES;202
11.4;REFERENCES;212
12;CHAPTER 6. DETERMINATION OF INHIBITORS;215
12.1;A. GENERAL;215
12.2;B. DETERMINATION OF INORGANIC SUBSTANCES;218
12.3;C. DETERMINATION OF PESTICIDES;235
12.4;D. DETERMINATION OF OTHER ORGANICS;247
12.5;REFERENCES;249
13;CHAPTER 7. THE IMMOBILIZED ENZYME;253
13.1;A. GENERAL;253
13.2;B. IMMOBILIZATION IN STARCH GEL;254
13.3;C. IMMOBILIZATION IN POLYACRYLAMIDE GELS;264
13.4;D. OTHER METHODS FOR IMMOBILIZING ENZYMES;276
13.5;E. COMMERCIAL AVAILABILITY OF IMMOBILIZED ENZYMES;279
13.6;F. FUTURE APPLICATIONS;280
13.7;REFERENCES;280
14;CHAPTER 8. USE OF AUTOMATION IN ENZYMIC ANALYSIS;283
14.1;A. GENERAL;283
14.2;B. INITIAL SLOPE METHOD;284
14.3;C FIXED CONCENTRATION (VARIABLE TIME) METHODS;286
14.4;D. FIXED TIME METHOD;290
14.5;REFERENCES;311
15;APPENDIX;313
16;AUTHOR INDEX;333
17;SUBJECT INDEX;347
18;OTHER TITLES IN THE SERIES IN ANALYTICAL CHEMISTRY;367