Hosseini / Ibrahim Novel Polymeric Biochips for Enhanced Detection of Infectious Diseases

1. Auflage 2016
ISBN: 978-981-10-0107-9
Verlag: Springer Singapore
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 63 Seiten, eBook

Reihe: SpringerBriefs in Forensic and Medical Bioinformatics

This book focuses on the creation and development of polymeric platforms (different compositions) from a specific polymer system. This system can be used as an adaptive technique for producing sensitive analytical devices, or for simple integration into existing bioanalytical tools in order to enhance the detection signal.

Zielgruppe

Research

Autoren/Hrsg.

Hosseini, Samira

Ibrahim, Fatimah

Inhaltsverzeichnis

1 Current optical biosensors in clinical practice
1.1 Biosensors
1.2 Enzyme-linked immunosorbent assay (ELISA)
1.3 Sandwich ELISA
1.3.1 Capture antibody immobilization
1.3.2 Washing procedure
1.3.3 Blocking step
1.3.4 Virus attachment
1.3.5 Primary antibody attachment
1.3.6 Secondary labeled antibody attachment and recording the signals
1.3.7 Evaluation of ELISA
1.4 Advantages and disadvantages of ELISA
1.5 Surface functionalization
References
2 Alternative chemical approach
2.1 Proposed chemical alternative
2.2 Characterization techniques
2.2.1 Morphology analysis by scanning electron microscopy (SEM)
2.2.2 Topography analysis by atomic force microscopy (AFM)
2.2.3 Water-in-air contact angle measurement
2.3 Dengue fever
References
3 Biochips Fabrication and surface characterization
3.1 Fabrication of the biochips
3.2 Morphology analysis of the biochips
3.3 Topography analysis of the biochips
3.4 Water-in-air contact angle measurement on the surface of biochips
References
4 Application of the biochips in dengue virus detection
4.1 Sandwich ELISA
4.2 Dengue virus detection in sandwich ELISA assay
4.3 Physical immobilization of the target analyte on the biochips and performance of the assay in dengue virus detection
4.3.1 Detection range study
4.3.2 Calibration of the assay
4.3.3 Evaluation of the assay
4.4 Covalent immobilization of the target analyte on the biochips and performance of the assay in dengue virus detection
4.5 Immobilization of the target analyte by using amine-bearing spacers
References
Summary
Appendix

Über Autor(innen)

Samira Hosseini obtained her BsC degree in Applied Physics at Tehran University, Iran and her MsC degree in Polymer Chemistry at Chemistry Department, University of Malaya, Malaysia. Currently she is finalizing her PhD degree in Biomedical Engineering at Department of Biomedical Engineering at University of Malaya, Malaysia. Her main areas of research are surface engineering, interface science, bioanalytical systems and polymeric materials.

Fatimah Ibrahim obtained her PhD degree in Biomedical Engineering from University of Malaya (UM) in 2005. She was a senior lecturer at Universiti Teknologi MARA and in 1999, she joined University of Malaya, and involved in setting up one of the first Departments of Biomedical Engineering in Malaysia. She did her Biological and Micro-Electromechanical Systems (BioMEMS) research attachment at University of Irvine California in 2010. She is currently a Professor in the Department of Biomedical Engineering and Head of Centre for Innovation in Medical Engineering (CIME) at UM. Her research interests are in detection and monitoring of diseases, physiological modeling and measurement, biosensors, BioMEMS and artificial intelligence applications in medicine.