Howard / Kaser Making and Using Antibodies

A Practical Handbook, Second Edition
2. Auflage 2013
ISBN: 978-1-4398-6910-9
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 458 Seiten

This second edition of a popular text provides clear guidance on all aspects of how to make and use antibodies for research, and commercial and industrial applications. All chapters are revised, updated, or expanded. New material in the book includes western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present clear descriptions of basic methods for making and using antibodies and describe basic laboratory techniques in detail. Each chapter includes introductory material, allowing for a better understanding of each concept. Practical examples are also included.

Autoren/Hrsg.

Howard, Gary C.

Kaser, Matthew R.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Immunologie
• Naturwissenschaften Biowissenschaften Biochemische Immunologie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Pharmazeutische Technologie

Inhaltsverzeichnis

Introduction. Antigens And Adjuvants. Polyclonal Antibodies. Monoclonal Antibodies. Purification and Charaterization. Bacteria and Phage. Quantitative Production of Monoclonal Antibodies. Aptamers. Modifying Antibodies. Westerns. Immunohistochemistry. Immune-Electron Microscopy. Flow Cytometry. ELISAs. Humanized Antibodies. Antibodies in the Future.