Iranzo / Roque Peptide and Protein Engineering

From Concepts to Biotechnological Applications
Erscheinungsjahr 2020
ISBN: 978-1-0716-0720-6
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 289 Seiten, eBook

Reihe: Springer Protocols Handbooks

This thorough book aims to present the methods that have enabled the success of peptides and proteins in a wide variety of applications. It opens with a section on chemical tools applied to the production or engineering of peptides and proteins, and concludes with a collection of chapters on biological approaches used to engineer structure and function in peptides and proteins. As a book in the Springer Protocols Handbooks series, chapters include the kind of detailed descriptions and tips necessary for successful results in practice.  Authoritative and practical, Peptide and Protein Engineering: From Concepts to Biotechnological Applications will be of great use to scientists in academia and industry seeking a better understanding of the emerging principles and methodologies in peptide and protein engineering.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Iranzo, Olga

Roque, Ana Cecília

Inhaltsverzeichnis

SEA Ligation is Accelerated at Midly Acidic pH: Application to the Formation of Difficult Peptide Junctions.- The Problem of Aspartimide Formation during Protein Chemical Synthesis Using SEA-Mediated Ligation.- Using the Interactive Tool of the Protein Chemical Synthesis Database.- Only a “Click” Away: Development of Arginine-Rich Peptide-Based Materials Using Click Chemistry.- Fluoroglycoproteins by Copper-Free Strain-Promoted Azide–Alkyne Cycloaddition.- Hybrid Silylated Peptides for the Design of Bio-Functionalized Materials.- Synthesis of Peptide-Oligoethylene Glycol (OEG) Conjugates for Multivalent Modification of Nanomaterials.- Phage Display Methodologies.- Whole-Bacterium Ribosome Display Selection for Isolation of Anti-Bacterial Affitins.- CIS Display: DNA-Based Technology as a Platform for Discovery of Therapeutic Biologics.- Building Scarless Gene Libraries in the Chromosome of Bacteria.- Streptavidin (Sav)-Based Artificial Metalloenzymes: Cofactor Design Considerations and Large Scale Expression of Host Protein Variants.- Integrated Bacterial Production of Large Combinatorial Libraries of Cyclic Oligopeptides and Functional Screening for the Identification of Chemical Rescuers of Pathogenic Protein Misfolding and Aggregation.- Site-Specific Incorporation of Non-Canonical Amino Acids by Amber Stop Codon Suppression in Escherichia coli .