Jiang / Shen / Gao Plant Protein Secretion

Methods and Protocols
2. Auflage 2024
ISBN: 978-1-0716-4059-3
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 250 Seiten

Reihe: Methods in Molecular Biology

This volume explores the latest developments in protein secretion research in plants, as compared to yeast and mammalian systems. The chapters in this book present a diverse and thorough perspective of the field and cover topics such as bioinformatic analysis, proteomic studies, ultrastructural analysis, and genetic screening methods. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, is a valuable resource for researchers and students in the field of plant biology, and will inspire further advancements in our understanding of protein secretion in plant cells and beyond.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Jiang, Liwen

Shen, Jinbo

Gao, Caiji

Wang, Xiangfeng

Inhaltsverzeichnis

Conventional and Unconventional Protein Secretion in Yeast and Animal Cells.- An Overview of Protein Secretion in Plant Cells.- Bioinformatic Analysis for Identifying Transcription Factors Involved in Protein Secretion and Vacuole Formation.- Using AlphaFold2 and Molecular Dynamics Simulation to Model Protein Recognition.- Proteomic Analysis of Secreted Vesicle Proteins from Plant Cells.- Extracellular Vesicle Isolation and Mass-Spectrometry-Based Proteomic Analysis in Polysaccharides Analysis of Cell Wall Using Carbohydrate Gel Electrophoresis.- Acetyl Bromide Method for Total Lignin Content Determination in Plant Biomass.- Utilizing Plant Protein Secretion System for Pharmaceutical Protein Production.- In Vitro Reconstitution of Plant COPII Vesicles.- Identification of Neighboring Proteins of Endosomal Regulators by Using TurboID-Based Proximity Labeling.- Quantification of Protein Dynamics in Growing Pollen Tubes via Whole Cell Kymograph Analysis.- Polar Localization Analysis of Anionic Phospholipids and Their Binding Proteins in Pollen Tubes.- Vacuum-Based Agroinfiltration for Transient Gene Expression in Seedlings.- Pharmaceutical Analysis in the Plant Endomembrane System.- Observation of Vacuoles in Developing Pollen Grain with Whole-Cell Electron Tomography.- Whole-Cell Electron Tomography Analysis of Vacuole Biogenesis.- Microscopy Analysis of Autophagosomal Structures in Plant Cells.- Electron Microscopy Analysis of Membraneless Condensates in Plant Cells.- High-Pressure Freezing and Low-Temperature Processing of Seeds for Electron Microscopy and Electron Tomography.- Determination of the Cryo-EM Structure of ATG9 from Genetic Screening of Factors in the Plant Protein Secretion.- Studying Autophagy and Senescence of Stigmatic Cells.