Jones / Lympany Allergy Methods and Protocols

2008
ISBN: 978-1-59745-366-0
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 138, 352 Seiten, eBook

Reihe: Methods in Molecular Medicine

Allergy: Methods and Protocols aims to assist the researcher in gaining insight into the molecular mechanisms involved in allergy by featuring an array of protocols. The protocols cover a range of disciplines including allergy, immunology, cell biology, and histology, and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, and T regulatory cells.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Jones, Meinir G.

Lympany, Penny

Inhaltsverzeichnis

List of chapters

1. Understanding the molecular mechanisms of allergy - Meinir Jones
2. T cell - primary culture from peripheral blood - Monika
Raulf-Heimsoth
3. Production of T cell lines - Helga Kahlert
4. Production of human T cell clones - Adrienne Verhoef
5. Mapping of human T-cell epitopes of allergens - Thomas Zeiler and
Tuomas Virtanen
6. Determining MHC restriction of T cell response - Mark Larche
7. Short term culture of CD8 cells and intracellular cytokine staining
- Beejal Vyas and Alistair Noble
8. Isolation, flow cytometric analysis and suppression assay of
CD4+CD25+ T regulatory cells - Hayley Jeal
9. Monocyte-derived dendritic cells as antigen presenting cells in T
cell proliferation and cytokine production - Sun-sang J Sung
10. Ultrasensitive ELISA for measurement of human cytokine responses in
primary culture - William P. Stefura, J. Darren Campbell, Renée
Douville, Monique J. Stinson, F. Estelle Simons, Allan B. Becker, and
Kent T. HayGlass
11. Quantification of human chemokine production in TLR-stimulated and
antigen-specific recall responses - Monique Stinson, Renee Douville,
Yuriy Lissitsyn, Melanie Blanchard, William Stefura, Estelle Simons,
Allan Becker, Peter Nickerson, Kevin Coombs, Kent HayGlass
12. Standardisation of allergen extracts - Jørgen Nedergaard Larsen and
Sten Dreborg
13. Immuno-electrophoresis for the characterisation of allergen
extracts - Gitte Nordskov Hansen and Jørgen Nedergaard Larsen
14. Conjugation of haptens - Ranulfo Lemus and Meryl H. Karol
15. Monoclonal antibodies - Helga Kahlert and Oliver Cromwell
16. Purification of antibodies - Per H Larsson and Anne Renström
17. Collection of air samples to quantify exposure to airborne
allergens - Susan Gordon
18. Assay of air sample eluates - Anne Renström and Susan Gordon
19. The halogen assay - a new technique for measuring airborne allergen
- Euan Tovey,Sandra De Lucca, Leanne Poulos and Tim O'Meara
20. Measurement of specific IgG anti-Fel d 1 antibodies - Meinir Jones
21. The facilitated-antigen binding (FAB) assay - a protocol to measure
allergen-specific inhibitory activity - James N Francis
22. Microscopic identification and purity determination of pollen
grains - Magdalena Rahl
23. Biopanning for the characterisation of allergen mimotopes -
Isabella Schöll and Erika Jensen-Jarolim
24. Identification of mast cells and mast cell subpopulations - Mark
Buckley and Andrew F Walls
25. Purification and characterisation of mast cell tryptase and chymase
- Alan R McEuen and Andrew F Walls
26. Experimental activation of mast cells and their pharmacological
modulation - Shaoheng He and Andrew F Walls
27. In situ hybridisation - Kayhan T. Nouri-Aria