Kamihira / Katakura / Ito Basic and Applied Aspects

Proceedings of the 21st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT), Fukuoka, Japan, November 24-27, 2008
1. Auflage 2010
ISBN: 978-90-481-3892-0
Verlag: Springer Netherlands
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 16, 446 Seiten

Reihe: Animal Cell Technology: Basic & Applied Aspects

Animal cell technology is a growing discipline of cell biology which aims not only to understand the structure, function and behavior of differentiated animal cells, but also to ascertain their ability to be used for industrial and medical purposes. Some of the major goals of animal cell technology include: the clonal expansion of differentiated cells, the optimization of their culture conditions, modulation of their ability for the production of medically and pharmaceutically important proteins and the application of animal cells to gene therapy, artificial organs and functional foods. This volume gives the readers a complete review of the present state-of-the-art research in Japan and other countries where this field is well advanced. The Proceedings will be useful to cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and to those working in either academic environments or in the biotechnology and pharmacy industries related to animal cell culture.

Autoren/Hrsg.

Kamihira, Masamichi

Katakura, Yoshinori

Ito, Akira

Inhaltsverzeichnis

1;Preface;6
2;Contents;8
3;Contributors;16
4;Purification of Hepatoblasts from Fetal Mouse Livers by Using a Temperature- Reversible Gelation Polymer and Their Application in Regenerative Medicine;32
5;Computer Aided Rational Design of Biochemical Systems;38
6;DNA Microarray in Nutrigenomics and Cancer Prevention Research: Elucidating Molecular Signatures of Cancer Preventive Phytochemicals;44
7;DNA Microarray Technology Evaluation of Effect of Dietary Intake of Crude Rice Glycosphingolipids in Mice;50
8;Integrated Pathway Analysis of Genome-Wide Expression Changes Associated with Serum- Free Suspension Adaptation of an Antibody- Producing Chinese Hamster Ovary ( CHO) Cell Line;57
9;Affordable Mab by Ensuring Low Cog;63
10;Evaluation of Neurotoxicity of Environmental Chemicals Using Neural Stem Cells and Neuroblastoma Cells;70
11;Integration of Endothelial Cell-Covered Hepatocyte Spheroids for Construction of Vascularized Liver Tissue;74
12;Characterization of Cell-Sized Vesicles Induced from Human Lymphoid Cell Lines;80
13;Dendrimer-Immobilized Culture Surface as a Tool to Promote Aggregate Formation of Anchorage- Dependent Cells;85
14;Advantage of Alagln as an Additive to Cell Culture Medium;92
15;Supplementation of Phosphatidylcholine Protects the Hybridoma Cells from Apoptosis Induced by SerumWithdrawal;99
16;Monoclonal Antibody Productivity of Hybridoma Cells Under Active Hypothermic Growth Condition;103
17;Efficient Selection of Cell Clones with Higher Productivity in the Production of Recombinant Human Monoclonal Antibodies;108
18;Continuous Cell Production from Three Dimensional Hematopoietic Microenvironment in Polyurethane Foam;114
19;A New Approach for Drug Discovery and Differentiation Study Using Cutting-Edge 3d Cell Culture System;120
20;Dimensional Cell Culture Dish for In Vivo-Like Cell Culture;128
21;Characterization of the Erythropoietin (EPO) Protein Production in the Recombinant Human Cultured Cells, in which the Exogenous EPO Gene was Introduced into Hypoxanthine Phosphoribosyl Transferase ( HPRT) Gene Locus;134
22;Development of Protein High Expression System by Using Fructose Metabolism;140
23;Controlling Fucosylation Levels of Antibodies with Osmolality during Cell Culture;145
24;Comparison of Immunoresponses Between Cecal Patch Cells and PeyerÌs Patch Cells Stimulated by Bacterial Components;150
25;Conjugate of ß-Lactoglobulin with Carboxymethyl Dextran Increased the Antigen- Specific IgG1 Production, While It Decreased IgE Production Compared with Native ß- Lactoglobulin;155
26;Anti-Inflammatory Action of Epigallocatechin- 3- Gallate at a Physiological Concentration through the 67- KDA Laminin Receptor in Lipopolysaccharide- Stimulated Raw264.7 Cells;160
27;Enhancement of Antibody Production using Solution after Antibody Purification: Application of Spent Medium for Antibody Production;166
28;Production of Human Monoclonal Antibodies Against FC Epsilon Receptor I Alpha by Combining In Vitro Immunization with Phage Display;172
29;Enhancement of Hepatocyte Function Through Heterotypic Cell- Cell Interactions Using E- Cadherin- Expressing NIH3T3 Cells;179
30;Magnetic Cell-Patterning for Tissue Engineering;184
31;Magnetic Force-Based Tissue Engineering of Skeletal Muscle for Bio- Actuator;190
32;Fabrication of Skeletal Muscle Tissue from C2C12 Myoblast Cell Towards the Use as Bio- Actuator;196
33;Retroviral Vectors Pseudotyped with Chimeric Vesicular Stomatitis Virus Glycoprotein for Antibody- Dependent Gene Transduction;203
34;Improvement of CPP-RBD for siRNA Delivery;209
35;Production of Recombinant Human EPO and EPO/ Fc Fusion Proteins by Chinese Hamster Ovary Cells;215
36;Development of Oviduct-Specific Gene Expression System for Transgenic Avian Bioreactor;221
37;Production of Therapeutic Proteins Composed of Seven Dominant Human T Cell Epitopes Derived from the Japanese Cedar Pollen Allergens;227
38;The Differentiation of C2C12 Cells to Myotube by Paraquat;233
39;Molecular Cloning and Characterization of a Novel Canine Sulfotransferase;238
40;In Vitro Analysis of the Interaction Between Human Intestinal Epithelial Cells and Macrophage- Like Cells;247
41;Role of Ubiquitination Promoted During Restimulation in the Induction of T CellAnergy;253
42;Expression Analysis of Senescence-Associated Genes: Their Possible Involvement in Diabetes;258
43;Study of Butyrate Signal Transduction Pathways in Rat Hepatic Stem- Like Cells;262
44;Growth Control of Mammalian Cells via a Human Artificial Chromosome Harboring a Chimeric Receptor;268
45;The Impact of Redox State on Regulation of the High- Affinity IgE Receptor Expression;274
46;The Characteristic of Chimeric Receptors Based on Erythropoietin Receptor;280
47;Anti-Cancer and Structure-Activity Relationship of Natural Polyacetylenes;286
48;Effect of Mongolian Medicinal Plant Stellera Chamaejasme on Chronic Leukemia Cells K562;291
49;Capsaicin Induced the Upregulation of Transcriptional and Translational Expression of Glycolytic Enzymes Related to Energy Metabolism in Human Intestinal Epithelial Cells;295
50;Effect of Novel Compounds from Thymelaea Hirsuta on Melanogenesis;298
51;Electrolyzed Reduced Water Prolongs Caenorhabditis elegans' Lifespan;301
52;Anti-Cancer Effects of Enzyme-Digested Fucoidan Extract from Seaweed Mozuku;306
53;The Downregulation of Mast Cell Activation Through the Suppression of the High- Affinity IgE Receptor Expression by Green Tea Catechin Egcg;312
54;Anti-Diabetes Effects of Hita Tenryou-Sui WaterR , a Natural Reduced Water;318
55;Suppressive Effect of ERW on Lipid Peroxidation and Plasma Triglyceride Level;325
56;Growth Suppression of HL60 and L6 Cells by Atomic Hydrogen;332
57;Insulin-Mimetic Activity of Inositol Derivatives Depends on Phosphorylation of PKC in L6 Myotubes;335
58;Enhancement of Phagocytic Activity by a Crude Polysaccharide from Tea ( Camellia sinensis) Extract;340
59;Effects of Lupeol on Melanoma In Vitro and In Vivo: Fundamental and Clinical Trials;346
60;The Effect of Secoisolariciresinol on 3t3-L1 Adipocytes and the Relationship Between Molecular Structure and the Activity;352
61;Detection of Anti-Allergic Factors in Strawberry Extracts;359
62;Anti-Melanogenic Activity of Yacon Leaves in Mouse Melanoma Cells;364
63;Cytokine Responses of Splenocytes of Female and Male Non- Obese Diabetic Mice Induced by Lactic Acid Bacteria;370
64;Development of a High-Throughput Evaluation System for Antiviral Activities of Food Factors By Using Bioinformatics;376
65;The Effects of Pesticides on Immune Cells;382
66;Co-Cultured Hepatocyte-Spheroid Chip Constructed Using the Microfabrication Technique;388
67;Micropatterned Hepatocyte Culture Using Microstencil Technique;395
68;Structural Features of Totally Designed Lipidic Materials for Small Interfering RNA Delivery System;401
69;Thin-Film Assembly of Totally Designed Lipidic Materials as Gene Carrier in Mouse Embryonic Neural Stem Cells: Thin- Film Assembly of Lipidic Material as Potential Gene Carrier;407
70;Transfection System Using a Cocktail of Totally Designed Lipidic Materials in Chinese Hamster Ovary Cells: Lipidic Cocktail as Potential Gene Carrier for CHO Cells;413
71;Size Dependent Heat Generation of Magnetic Nanoparticles Under AC Magnetic Field for Cancer Therapy;419
72;Functional Evaluation of Anticancer Drugs in Human Leukemia Cells Based on Metabolic Profiling Technique;426
73;Extracts from the Root of Platycodon grandiflorum Modulate Induction of Inflammatory Changes in Coculture of Adipocytes and Macrophages;432
74;Position Specific Incorporation of Nonnatural Amino Acids into Proteins In Vivo;438
75;Novel Protease Inhibitor Inhibiting Proliferation of Influenza Virus: Purification and cDNA Cloning of a Novel Protease Inhibitor Secreted by MDCK Cells;443