Kawasaki / Yoshioka Plant Immunity

Methods and Protocols
Erscheinungsjahr 2026
ISBN: 978-1-0716-5138-4
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 266 Seiten

Reihe: Methods in Molecular Biology

This volume explores plant immunity through protocols for recently established genetic and biochemical techniques to elucidate immune mechanisms mediated by pattern-recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). The book covers the identification of PRRs, their associated downstream immune regulators, PRR-ligand interaction-based immune responses, identification of NLR complex components, pan-NLRome analysis, resistosome assays, and cell death evaluation. Additionally, it presents protocols for co-transcriptome analysis of plant-pathogen interactions, single-nucleus multi-omics, metabolite imaging, and bioluminescence-based quantification of pathogen growth, as well as genome editing tools that contribute to the development of disease-resistant plants. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, serves as an ideal guide for researchers aiming to accelerate ongoing research and advances in the field of plant immunity.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Kawasaki, Tsutomu

Yoshioka, Hirofumi

Inhaltsverzeichnis

Lumi-Map, a Luciferase Bioluminescence Screen of Mutants Combined with MutMap Allows Rapid Dissection of Plant Immune Signaling Pathways.- Identification of Phosphorylation and Protein Interactions of Plant Pattern Recognition Receptors Through Immunoprecipitation.- Plant-Based Ligand-Depletion Binding Assay for Assessing Pattern Recognition Receptor-Ligand Interactions.- Plate Reader-Based Quantification of Cellular Calcium Dynamics in Immune Responses Using the Genetically-Encoded Calcium Indicator GCaMP.- Visualization of Calcium Decoding via a FRET-Based Reporter of Calcium-Decoder Conformation.- Visualization and Quantification of Apoplastic ROS by Plant Immune NADPH Oxidase Utilizing Chemiluminescence.- Detection of Phosphorylated NADPH Oxidase by Antibody During Plant Immune Responses.- The Application of RenSeq for Pan-NLRome Analysis.- Blue Native-PAGE Assay of NLR Protein Complexes Combined with Genetic Complementation in Nicotiana benthamiana.- Quantification of NLR-Induced Cell Death Using Rice Protoplasts.- Split-TurboID-Based Proximity Labeling for Probing the Composition of Spatiotemporally Defined Protein Complexes In Planta.- Virus-Induced Gene Silencing for Identifying Autoimmune Regulators in Arabidopsis.- Bioinformatics Workflow for Co-Transcriptome Analysis of Plant-Bacterial Interactions.- Nuclei Isolation from Mature Leaves of Arabidopsis thaliana Infected by Bacterial Pathogens for Single-Nucleus Multiomics.- Multimodal Analysis of Phytoalexin Synthesis in Arabidopsis by Mass Spectrometry Imaging and Fluorescent Microscopy.- Bioluminescence-Based Growth Quantification of the Phytopathogenic Bacterium Pseudomonas syringae pv. tomato DC3000.- Genome Editing for Developing Disease-Resistant Plants.