Kioussi Odontogenesis

Methods and Protocols
Erscheinungsjahr 2012
ISBN: 978-1-61779-860-3
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 887, 194 Seiten, eBook

Reihe: Methods in Molecular Biology

Only in recent times has the possibility of growing and implanting replacement teeth, made from one’s own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be studied in a range of vertebrates, from zebrafish to mice, so that evolutionarily conserved network kernels, which will define the cellular states of generic vertebrate tooth development, can be recognized.  In Odontogenesis: Methods and Protocols , experts in the field examine techniques to approach this burgeoning field.  This detailed volume includes chapters on the detection of “tooth development” gene expression, both at the RNA and protein level, current approaches to the manipulation of gene expression levels and subsequent analysis of tooth phenotypes, as well as chapters concerning current efforts to get living tooth implants working without waiting for a full understanding of the developmental pathways at the molecular level.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.   Practical and easy to use, Odontogenesis: Methods and Protocols aims to help researchers move forward toward the ultimate goal of getting a “bioengineered tooth” into the patient’s mouth.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Kioussi, Chrissa

Inhaltsverzeichnis

Histological Analysis of the Embryonic and Adult Tooth.- Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization.- Determination of Gene Expression Patterns by In Situ Hybridization in Sections.- Immunohistochemistry and Detection of Proliferating Cells by BrdU.- Detection of Apoptosis by TUNEL Assay.- Use of siRNA in Dental Tissue-Derived Cell Cultures: Integrin Knockdown in Fibroblasts.- Organ Cultures and Kidney-Capsule Grafting of Tooth Germs.- Evaluation of Skull and Tooth Morphology and Mineralization Using High-Resolution X-Ray Tomography.- Electron Microscopy.- Deoxyoligonucleotide Microarrays for Gene Expression Profiling in Murine Tooth Germs.- Lineage Differentiation of Mesenchymal Stem Cells from Dental Pulp, Apical Papilla, and Periodontal Ligament.- In Vivo Transplantation and Tooth Repair.- Methods to Validate Tooth-Supporting Regenerative Therapies.- Generation of a Bioengineered Tooth by Using a Three-Dimensional Cell Manipulation Method (Organ Germ Method).- In Vitro Studies on Odontogenic Tumors.- Whole Mount Immunohistochemistry and In Situ Hybridization of Larval and Adult Zebrafish Dental Tissues.