Kovalchuk Plant Epigenetics

Methods and Protocols
2. Auflage 2017
ISBN: 978-1-4899-7708-3
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1456, 253 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding RNAs, changes in DNA methylation, and histone modifications in plants. Chapters detail protocols with different degrees of complexity, and describe bioinformatics approaches for data processing and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Kovalchuk, Igor

Inhaltsverzeichnis

1. Chromatin Immunoprecipitation
Protocol for Histone Modifications and Protein-DNA Binding Analyses in ArabidopsisWanhui You, Stéphane Pien, and Ueli
Grossniklaus 2.
Chromatin Conformation Capture-Based Analysis of Nuclear ArchitectureStefan Grob and Ueli Grossniklaus 3.
Metaanalysis of Genome-Wide Chromatin DataJulia
Engelhorn and Franziska Turck 4.
Localization of Mirnas by In Situ Hybridization in Plants using Conventional
Oligonucleotide ProbesSara
Hernández-Castellano, Geovanny I. Nic-Can, Clelia
De-La-Peña 5.
COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of
Locus-Specific Changes in Methylation Patterns Andriy
Bilichak and Igor Kovalchuk 6.
Analysis of Global Genome Methylation Using the Cytosine-Extension AssayAndriy
Bilichak and Igor Kovalchuk  7.
In Situ Analysis Of DNA Methylation in PlantsPalak
Kathiria and Igor Kovalchuk < 8. Analysis of DNA Hydroxymethylation in Arabidopsis Using
Colorimetric Assay Andrey Golubov and Igor Kovalchuk 9. Analysis of DNA Cytosine Methylation Patterns using
Methylation Specific Amplification Polymorphism (MSAP)María Ángeles Guevara, Nuria De María, Enrique Sáez-Laguna,
María Dolores Vélez, María Teresa Cervera, and José Antonio Cabezas 10. Differentially Methylated
Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify
Dmrs in Uncharacterized GenomesPavlina Sasheva Ueli Grossniklaus 11. Analysis of Small RNA Populations Using
Hybridization to DNA Tiling Arrays Martine
Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David
Baulcombe, and Vincent Colot  12. Northern Blotting Techniques for Small RNAsTodd
Blevins  13. Stem-Loop QRT-PCR for the Detection of Plant
MicrornasErika Varkonyi-Gasic 14.
Profiling New Small RNA SequencesMasayuki Tsuzuki and Yuichiro Watanabe 15.
Small RNA Library Preparation and Illumina Sequencing in PlantsAndriy
Bilichak, Andrey Golubov, and Igor Kovalchuk 16.
Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow