Kowalska / Sajewicz / Sherma Planar Chromatography – Mass Spectrometry

Erscheinungsjahr 2015
ISBN: 978-1-4987-0589-9
Verlag: CRC Press
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 406 Seiten

Reihe: Chromatographic Science Series

This book focuses on a relatively new approach to chemical analysis in general and to separation science in particular. It covers the combination of thin-layer chromatography (TLC) and high performance TLC (HPTLC), one of the most simple, cost-effective, and well-performing techniques for determining complex mixtures of compounds along with mass spectrometry (MS), a sophisticated and relatively expensive spectrometric technique that enables rapid identification of separated chemical species. In addition to that, the book covers electrophoretic – MS methods and applications.

Zielgruppe

This book is intended for separation science practitioners at all levels of professional expertise (from junior and senior class undergraduate students to graduate school academics as well as industrial and regulatory analytical chemists). It would be particularly useful to those working in traditional Chinese and Indian herbal medicine, food analysis, and forensic analysis; as well as to clinical laboratory personnel working in the medical diagnostics area.

Autoren/Hrsg.

Kowalska, Teresa

Sajewicz, Mieczyslaw

Sherma, Joseph

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Naturwissenschaften Chemie Chemie Allgemein
• Naturwissenschaften Chemie Analytische Chemie

Inhaltsverzeichnis

SECTION I. Introduction. Applicability of commercial chromatographic thin layers for mass spectrometric detection. CAMAG TLC-MS interface. Principles of mass spectrometry imaging applicable to thin-layer chromatography. Principles of mass spectrometry applicable to electrophoretic techniques. Selection of ionization methods of analyses in the TLC-MS techniques. Interfacing TLC with laser based ambient mass spectrometry. TLC-MS analysis using solvent elution of compounds from chromatographic media. Recording of mass spectra from miniaturized layers (UTLC-MS). SECTION II. Analysis of proteins and peptides - optimization of the chromatographic separation in the context of different mass spectrometric methodologies. Strategies of coupling planar chromatography to HPLC/MS. Drug analysis by TLC-DESI MS (TLC- imaging) - hyphenated technique. Application of TLC FAPA MS in medicine and forensic sciences. TLC - MALDI - MS for the analysis of lipids. Application of TLC-MS to drug photodegradation studies. Combination of thin-layer chromatography with electrospray ionization- and laser desorption ionization-mass spectrometric techniques for screening of organic compounds. Application of TLC-MS to analysis of drugs of abuse. TLC-MS analysis of carotenoids, triterpenoids and flavanols in plant extracts and food supplements. TLC - MALDI MS of carbohydrates. Spontaneous peptidization of amino acids traced by means of TLC-MS.

Über Autor(innen)

Teresa Kowalska and Mieczyslaw Sajewicz are affiliated with the Institute of Chemistry, University of Silesia, Katowice, Poland.

Joseph Sherma is a professor in the Department of Chemistry at Lafayette College, Easton, Pennsylvania, USA.