Krysan / Moye-Rowley Antifungal Drug Resistance

Methods and Protocols
Erscheinungsjahr 2023
ISBN: 978-1-0716-3155-3
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 250 Seiten

Reihe: Springer Protocols

This detailed volume aims to elucidate the molecular mechanisms that underlie antifungal resistance. The book highlights methods to identify and characterize antifungal activity, to define and characterize strains with altered responses to antifungal drugs, to investigate the genetic and molecular mechanisms of these alterations of antifungal drug susceptibility, and, finally, to approach the study of these processes in animal models of fungal infection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Antifungal Drug Resistance: Methods and Protocols serves as an ideal resource for the field and a guide for investigators to study this important translational aspect of fungal biology.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Krysan, Damian J.

Moye-Rowley, W. Scott

Inhaltsverzeichnis

Antifungal Susceptibility of Yeasts and Filamentous Fungi by CLSI Broth Microdilution Testing.- Luciferase-Based High Throughput Screen with Aspergillus fumigatus to Identify Antifungal Small Molecules.- A Dual-Readout High Throughput Screening Assay for Small Molecules Active Against Aspergillus fumigatus .- Antifungal Susceptibility Testing and Drug Discovery in the Dimorphic Fungus Histoplasma capsulatum .- A 384-Well Microtiter Plate Model for Candida Biofilm Formation and Its Application to High Throughput Screening.- Protocols for Measuring Tolerant and Heteroresistant Drug Responses of Pathogenic Yeasts.- Genome-Wide Identification of Variants Associated with Antifungal Drug Resistance.- Copy Number Variation and Allele Ratio Analysis in Candida ablicans Using Whole Genome Sequencing Data.- Drug Target Elucidation through Isolation and Analysis of Drug-Resistant Mutants in Cryptococcus neoformans .- Chemical-Genetic Approaches for Exploring Mode of Action of Antifungal Compounds in the Fungal Pathogen  Candida albicans .- Characterizing Candida glabrata Pdr1, a Hyperactive Transcription Factor Involved in Azole Resistance.- Heterologous Expression of Candida Antifungal Target Genes in the Model Organism Saccharomyces cerevisiae .- Conditional Protein Depletion in the Analysis of Antifungal Drug Resistance in Candida glabrata .- Transport Across Membranes: Techniques for Measuring Efflux in Fungal Cells.- Transport Across Membranes: Techniques for Measuring Drug Import in Fungal Cells.- Analysis of Candida Antifungal Resistance Using Animal Infection Models.- Using Bioluminescence to Image Candida glabrata Urinary Tract Infections in Mice.