Lacorazza Cellular Quiescence

Methods and Protocols
1. Auflage 2018
ISBN: 978-1-4939-7371-2
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1686, 303 Seiten, eBook

Reihe: Methods in Molecular Biology

This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful  Methods in Molecular Biology  series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls. Authoritative and practical,  Cellular Quiescence: Methods and Protocols  offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Lacorazza, H. Daniel

Inhaltsverzeichnis

Molecular Regulation of Cellular Quiescence: A Perspective from Adult Stem Cells and Its Niches.- An In Vitro   Model of Cellular Quiescence in Primary Human Dermal Fibroblasts.- Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining.- Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells.- Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System.- Determination of Histone 2B-Green Fluorescent Protein (GFP) Retention in Intestinal Stem Cells.- Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation.- Cell Cycle Analysis by Mass Cytometry.- Preparation and Analysis of  Saccharomyces cerevisiae  Quiescent Cells.- Identifying Quiescent Stem Cells in Hair Follicles.- Single EDL Myofiber Isolation for Analyses of Quiescent and Activated Muscle Stem Cells.- Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence.- Retroviral Transduction of Quiescent Murine Hematopoietic Stem Cells.- Analysis of Murine Hematopoietic Stem Cell Proliferation During Inflammation.- A Facile, In Vitro 384-Well Plate System to Model Disseminated Tumor Cells in the Bone Marrow Microenvironment.- Distinguishing States of Arrest: Genome-Wide Descriptions of Cellular Quiescence Using ChIP-Seq and RNA-Seq Analysis.- Analysis of lncRNA-Protein Interactions by RNA-Protein Pull-Down Assay and RNA Immunoprecipitation (RIP).- Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in Quiescent Cells.- Genome-Wide Identification of Transcription Factor Binding Sites in Quiescent Adult Neural Stem Cells.- Study Quiescence Heterogeneity by Coupling Single-Cell Measurements and Computer Modeling.