Lepenies Carbohydrate-Based Vaccines

Methods and Protocols
2015
ISBN: 978-1-4939-2874-3
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1331, 255 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Carbohydrate-Based Vaccines: Methods and Protocols aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Lepenies, Bernd

Inhaltsverzeichnis

Carbohydrate-based Vaccines: An Overview.- Glycans as Vaccine Antigens and Adjuvants: Immunological Considerations .- The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens.- Antibody-carbohydrate Recognition from Docked Ensembles using the AutoMap Procedure.- Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens.- Murine Whole-Blood Opsonophagocytosis Assay to Evaluate Protection by Antibodies Raised Against Encapsulated Extracellular Bacteria.- Determination of N -linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility.- N- Glycosylation Fingerprinting of Viral Glycoproteins by xCGE-LIF.- Temporary Conversion of Protein Amino Groups To Azides: A Synthetic Strategy For Glycoconjugate Vaccines.- Gold Nanoparticles as Carriers For Synthetic Glycoconjugate Vaccines.- Identification and Characterization of Carbohydrate-Based Adjuvants.- Characterization of Carbohydrate Vaccines by NMR Spectroscopy Francesco Berti and Neil Ravenscroft.- Characterisation of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods.- Glycoconjugate Vaccines: The Regulatory Framework.