Magee Selected Readings in Chromatography

The Commonwealth and International Library: Selected Readings in Analytical Chemistry
1. Auflage 2013
ISBN: 978-1-4831-8146-2
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 138 Seiten, Web PDF

Selected Readings in Chromatography describes the series of extractions by adsorption or partition involved in chromatography. This book discusses the counter-current process that is analogous to fractional distillation. The text describes the use of thin-layer chromatography that combines the advantages of column chromatography with the rate of speed achieved in paper chromatography. The book explains chromatography with electrophoresis when used with paper or with amino acids. The text analyzes the phenomena of an ion exchanger first observed by Thompson in 1845, as well as the two types of ion-exchange resins, namely, anion exchangers and cation exchangers. Experiments conducted verify the theory of the partition columns which has been extended to cover a compressible mobile phase. The book also compares the two methods of calculating the height equivalent of a theoretical plate in the partition columns; the book also discusses the factor influencing the degree of separation. The book describes gas-liquid partition columns during separation of volatile fatty acids from formic acid to dodecanoic acid. This book is intended for students of sixth formers, of technical schools, and undergraduates of biochemistry or analytical chemistry.

Autoren/Hrsg.

Magee, R. J.

Inhaltsverzeichnis

1;Front Cover;1
2;Selected Readings in Chromatography;4
3;Copyright Page;5
4;Table of Contents;6
5;Preface;8
6;Chapter 1. Types of Chromatography;10
6.1;The discovery of chromatography;10
6.2;The principles of chromatography;11
6.3;Nomenclature;15
6.4;The work of Tswett;16
6.5;Zur Chemie des Chlorophylls. Cber Phylloxanthin, Phyllocyanin und die Chlorophyllane;17
6.5.1;Die Zusammensetzung des Chlorophylls. Die Chlorophylline.;20
6.5.2;Partition chromatography;22
6.5.3;The "theoretical plate" analogy;23
6.5.4;Gas-liquid chromatography [GLC];26
6.6;151. A New Form of Chromatogram Employing Two Liquid Phases;27
6.6.1;Introduction;27
6.6.2;I. A theory of chromatography;29
6.6.3;Discussion;36
6.6.4;2. Application to the micro-determination of the higher monoamino-acids in proteins;37
6.7;Summary;44
6.8;References;45
6.9;Questions and Exercises;46
6.10;Suggested further reading;46
7;Chapter 2. Thin-layer Chromatography;50
7.1;Discussion;64
7.2;Acknowledgment;67
7.3;Literature cited;67
7.4;Questions and Exercises;68
7.5;Suggested further reading;68
8;Chapter 3. Chromatography with Electrophoresis;69
8.1;Ionic conductance;69
8.2;Paper electrophoresis;70
8.3;Electrophoresis of amino-acids;71
8.4;Electrophoresis combined with paper chromatography;72
8.5;Discussion;78
8.6;Summary;79
8.7;Questions and Exercises;80
8.8;Suggested further reading;80
9;Chapter 4. Ion-exchange Chromatography;81
9.1;Ion-exchange resins;81
9.2;Ion-exchange chromatography;83
9.3;Separation of the lanthanide and actinide elements;84
9.4;Discussion;102
9.5;Suggested further reading;109
10;Chapter 5. Gas Chromatography;110
10.1;The present situation;110
10.2;Capillary columns;113
10.3;A theory of gas-liquid partition chromatography;116
10.4;Factors influencing the degree of separation;123
10.5;Apparatus;126
10.6;Application to the separation of the volatile fatty acids;131
10.7;Results;132
10.8;Summary;143
10.9;References;143
10.10;Suggested further reading;144