Matson Microarray Methods and Protocols

1. Auflage 2009
ISBN: 978-1-4200-4666-3
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 216 Seiten

A Step-by-Step Guide to Present and Future Uses of Microarray Technology

Microarray technology continues to evolve, taking on a variety of forms. From the spotting of cDNA and the in situ synthesis of oligonucleotide arrays now come microarrays comprising proteins, carbohydrates, drugs, tissues, and cells. With contributions from microarray experts in both academia and industry, Microarray Methods and Protocols is a turn-by-turn roadmap through the processes necessary to perform a successful microarray experiment.

This easy to use book addresses the fundamental aspects of preparing and processing microarrays and bead arrays, labeling, and detection. It also includes a detailed How it Works section that discusses the underlying principles of a number of techniques. Troubleshooting guides offer additional advice for the successful performance of more than 100 protocols in 10 chapters that cover work involving nucleic acids, proteins, carbohydrates, and lectins.

--Concise and Well-Organized--

With a focus on the preparation and use of microarrays of biomedical relevance, the text describes a variety of microarray formats useful in the assessment of human disease and in genomic and proteomic research. This authoritative resource provides detailed information regarding sample preparation, labeling, array construction processes, substrate chemistry, array printing, and quality control.

Originating with the glass microscope slide and biochip, microarray technology is now pressing onward into the nanotechnology frontier. This book is the all-inclusive manual scientists need to take microarray research to the next level of discovery.

Zielgruppe

Academic, clinical, and industry scientists; established investigators, post-doctoral fellows, graduate students, and advanced undergraduates.

Autoren/Hrsg.

Matson, Robert S.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen

Inhaltsverzeichnis

Introduction to Microarray Technologies, Robert S. Matson
Nucleic Acid Sample Preparation, Robert S. Matson
Solid-Phase Substrates for Nucleic Acid Microarrays, Robert S. Matson
Protein Sample Preparation for Microarrays, Robert S. Matson
Solid-Phase Chemistries for Protein Microarrays, Robert S. Matson
Protein Microarrays: The Link between Genomics and Proteomics, Persis P. Wadia and David B. Miklos
Bead Arrays: An Introduction to Multiplexed Bead-Based Assays for Proteins, Yong Song
Carbohydrate Arrays, Denong Wang
Lectin Microarrays, Masao Yamada
Printing Methods, Todd Martinsky

Appendix A: Microarray Reagent, Materials, and
Equipment Sources

Appendix B: Image Analysis
Index