E-Book, Englisch, 115 Seiten
miRNA Regulation of the Translational Machinery
1. Auflage 2009
ISBN: 978-3-642-03103-8
Verlag: Springer
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)
E-Book, Englisch, 115 Seiten
ISBN: 978-3-642-03103-8
Verlag: Springer
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)
This book is dedicated to understanding how miRNAs affect translation. It includes chapters representing work in plants and Caenorhabditis elegans, the biological systems that originally led to the discovery of small interfering RNAs.
Autoren/Hrsg.
Weitere Infos & Material
1;Preface;6
2;Contents;8
3;Contributors;9
4;Understanding How miRNAs Post- Transcriptionally Regulate Gene Expression;11
4.1;1.1 Introduction;12
4.2;1.2 miRNA Biogenesis;15
4.3;1.3 miRNA-Mediated Regulation of Eukaryotic Gene Expression;17
4.4;1.4 Summary;25
4.5;References;25
5;Translational Control of Endogenous MicroRNA Target Genes in C. elegans;31
5.1;2.1 Introduction;32
5.2;2.2 lin-4 and let-7 miRNAs in C. elegans Development;32
5.3;2.3 Polysome Profiling as an Assay to Assess the Translational State of mRNAs;34
5.4;2.4 MicroRNA-Mediated Gene Regulation in C. elegans: The Early View;35
5.5;2.5 MicroRNA Mediated Gene Regulation in Other Model Organisms;36
5.6;2.6 The let-7 miRNA Extensively Interacts with Translation Factors;40
5.7;2.7 Polysome Profiling Confirms Translational Repression at the Initiation Step in C. elegans;41
5.8;2.8 Inhibition of Translation Initiation and Transcript Degradation Both Depend on the GW182 Proteins AIN-1 and AIN- 2;44
5.9;2.9 Conclusions and Future Perspectives;46
5.10;References;47
6;Translational Inhibition by MicroRNAs in Plants;51
6.1;3.1 Introduction;52
6.2;3.2 miRNA and siRNA Biogenesis;52
6.3;3.3 ARGONAUTE Proteins;54
6.4;3.4 Translational Inhibition by Small RNAs is Common in Plants;55
6.5;3.5 Roles of miRNA-Mediated Translational Inhibition in Plants;58
6.6;3.6 Conclusion;62
6.7;References;63
7;Regulation of p27kip1 mRNA Expression by MicroRNAs;68
7.1;4.1 Introduction;68
7.2;4.2 miRNAs in Cancer;69
7.3;4.3 Role of p27 in Tumor Progression;71
7.4;4.4 Regulation of p27 Expression: Role of miRNAs;72
7.5;4.5 Conclusions;75
7.6;References;75
8;The Inhibitory Effect of Apolipoprotein B mRNA- Editing Enzyme Catalytic Polypeptide- Like 3G ( APOBEC3G) and Its Family Members on the Activity of Cellular MicroRNAs;80
8.1;5.1 The Antiviral Activity of APOBEC3G;81
8.2;5.2 The Translational Suppression Function of MicroRNA;82
8.3;5.3 The Role of P-bodies in Regulating the Function of MicroRNAs;84
8.4;5.4 APOBEC3G Counteracts miRNA-Mediated Repression of Protein Translation;85
8.5;5.5 Conclusion Remarks;87
8.6;References;88
9;MicroRNA-Mediated mRNA Deadenylation and Repression of Protein Synthesis in a Mammalian Cell- Free System;93
9.1;6.1 Introduction;94
9.2;6.2 Cell-Free Systems that Recapitulate RNA Silencing;95
9.3;6.3 Cell Extract Preparation Methodology;95
9.4;6.4 Processing of Pre-miRNA;96
9.5;6.5 In Vitro Translation Assay;97
9.6;6.6 Let-7-Mediated mRNA Deadenylation;100
9.7;6.7 Poly(A) tail and miRNA-Mediated Repression;102
9.8;6.8 Poly(A) tail and Post-Transcriptional Regulation;103
9.9;6.9 Concluding Remarks;103
9.10;References;104
10;miRNA Effects on mRNA Closed-Loop Formation During Translation Initiation;106
10.1;7.1 Introduction;106
10.2;7.2 Analysing Translational Repression by an miRNA;109
10.3;7.3 Towards a Model for miRNA-Mediated Translational Control;114
10.4;7.4 Concluding Remarks;116
10.5;Note Added in Proof;116
10.6;References;116
11;Index;120
