Niessen Liquid Chromatography-Mass Spectrometry, Third Edition

3. Auflage 2012
ISBN: 978-1-4200-1454-9
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 632 Seiten

Reihe: Chromatographic Science Series

A constructive evaluation of the most significant developments in liquid chromatography–mass spectrometry (LC–MS) and its uses for quantitative bioanalysis and characterization for a diverse range of disciplines, Liquid Chromatography–Mass Spectrometry, Third Edition offers a well-rounded coverage of the latest technological developments and applications. As the technology itself has matured into a reliable analytical method over the last 15 years, the most exciting developments occur in LC–MS augments research into new applications. This edition places a stronger emphasis than previous editions on the impact of LC–MS methods, dedicating two-thirds of the text to small-molecule and biomolecular applications such as proteomics, pharmaceutical drug discovery and development, biochemistry, clinical analysis, environmental studies, and natural products research. Supported by the most relevant literature available, each chapter examines how the strategies, technologies, and recent advances—from sample pretreatment to data processing—in LC–MS helped to shape these disciplines. Featuring new chapters and extensive revisions throughout the book, Liquid Chromatography–Mass Spectrometry, Third Edition continues to provide scientists with a definitive guide and reference to the most important principles, strategies, and experimental precedents for applying LC–MS to their research.

Zielgruppe

Students, researchers, and professionals in separation science, analytical chemistry, organic chemistry, industrial process chemistry, drug discovery and development, pharmacokinetics, biochemical, biotechnology, proteomics, natural product research, and applied environmental chemistry.

Autoren/Hrsg.

Niessen, Wilfried M.A.

Fachgebiete

• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie

Inhaltsverzeichnis

INTRODUCTION

Liquid Chromatography and Sample Pretreatment

Mass Spectrometry

TECHNOLOGY

Strategies in LC–MS Interfacing

History of LC–MS Interfaces

Interfaces for Atmospheric-Pressure Ionization

Atmospheric-Pressure Ionization

APPLICATIONS: SMALL MOLECULES

LC–MS Analysis of Pesticides

Environmental Applications of LC–MS

LC–MS in Drug Discovery and Development

LC–MS in Drug Metabolism

Quantitative Bioanalysis Using LC–MS

Clinical Applications of LC–MS

LC–MS Analysis of Steroids

LC–MS in Food Safety Analysis

LC–MS Analysis of Plant Phenols

APPLICATIONS: BIOMOLECULES

LC–MS analysis of Proteins

LC–MS analysis of Peptides / Enabling Technology

LC–MS in Proteomics

LC–MS for Identification of Post-Translational Modifications

LC–MS Analysis of Oligosaccharides

LC–MS Analysis of Lipids and Phospholipids

LC–MS Analysis of Nucleic Acids INDEX