O'Connor / Hames Proteomics

Methods Express
Erscheinungsjahr 2007
ISBN: 978-1-907904-43-1
Verlag: Scion Publishing
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 300 Seiten, Web PDF

Reihe: Methods Express

Proteomics: Methods Express identifies the most powerful new technologies and presents them in a way that allows their robust implementation.

The focus is on proteomic methods and strategies that are reliable and of general applicability. Each chapter presents descriptions of what can, and cannot, be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house.

Every chapter discusses the merits and limitations of various approaches then provides tried-and-tested protocols with hints and tips for success and troubleshooting for when things go wrong.

Autoren/Hrsg.

O'Connor, C. David

Hames, B. David

Inhaltsverzeichnis

1. Sample preparation and subcellular fractionation approaches: purification of membranes and their microdomains for mass spectrometry analysis

Yan Li, Phil Oh, and Jan E. Schnitzer, Sidney Kimmel Cancer Center

2. An isotope-coding strategy for quantitative proteomics

Xian Chen, Dept of Biochemistry and Biophysics, University of N. Carolina at Chapel Hill

3. Gel-based approaches

Stuart Cordwell and Ben Crossett, both at School of Molecular and Microbial Biosciences, University of Sydney, and Melanie Y. White, Minomic Pty Ltd

4. Peptide sorting by reverse-phase diagonal chromatography

Kris Gevaert and Joel Vandekerckhove, Faculty of Medicine and Health Sciences, Ghent University

5. Mass spectrometry strategies for protein identification

David R. Goodlett, University of Washington and Garry L. Corthals, University of Turku

6. Desorption electrospray ionization: proteomics studies by a method that bridges ESI and MALDI

Zoltan Takats, Justin M. Wiseman, Demian R. Ifa and R.Graham Cooks, all at Dept of Chemistry, Purdue University

7. Analysis of cellular protein complexes by affinity purification and mass spectrometry

Tilmann Buerckstuemmer and Keiryn L. Bennett, both at Research Center for Molecular Medicine of the Austrian Academy of Sciences

8. Clinical proteomic profiling and disease signatures

Rosamonde E. Banks, David A. Cairns, David N. Perkins and Jennifer H. Barrett, all at Cancer Research UK Clinical Centre, St James' s University Hospital, Leeds

9. Characterization of post-translational modifications: undertaking the phosphoproteome

W. Andy Tao, Purdue University; Bernd Bodenmiller and Ruedi Aebersold, both Institute for Molecular Systems Biology, Federal Institute of Technology, Zurich

10. Protein microarray technologies

Chien-Sheng Chen, Sheng-Ce Tao, and Heng Zhu, Dept of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine

11. Intelligent mining of complex data: challenging the proteomic bottleneck

Dan Bach Kristensen, Maxygen and Alexandre Potelejnikov, Proxeon

12. Bioinformatic approaches in proteomics

Sandra Orchard and Henning Hermjakob, both European Bioinformatics Institute, Hinxton

List of suppliers

Index