Opara Cell Microencapsulation

Methods and Protocols
1. Auflage 2017
ISBN: 978-1-4939-6364-5
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1479, 366 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume provides a unique forum to review cell microencapsulation in a broad sense by exploring various cell types that have been encapsulated for different purposes, different approaches and devices used for microencapsulation, the biomaterials used in cell microencapsulation, the challenges to the technology, and the current status of its application in different clinical situations. This book is divided in five sections: Section I is an introductory part that discusses historical developments of the technology and its current challenges, as well as the various applications of cell microencapsulation; Section II discusses the main approaches and devices currently used in cell microencapsulation; Section III presents an overview of the various polymeric materials currently in use for cell microencapsulation and the enabling technologies to either monitor or enhance encapsulated cell function; Section IV gives specific examples of the methods used to encapsulate various cell types; and Section V provides an overview of the different clinical situations in which cell microencapsulation has been applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Cell Microencapsulation: Methods and Protocols is a valuable reference for researchers, engineers, clinicians, and other healthcare professionals, as well as food technologists who will find detailed descriptions of methods for the microencapsulation of specific cell types and their current of potential clinical and industrial applications. This volume also includes detailed information about the design and manufacture of different devices including large-scale production devices for use in cell microencapsulation.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Opara, Emmanuel C.

Inhaltsverzeichnis

Part I Introduction

1. Historical Perspectives and Current Challenges in Cell Microencapsulation Paul de Vos2. Applications of Cell MicroencapsulationEmmanuel C. OparaPart II Approaches to Cell Microencapsulation3. Cell Microencapsulation: Dripping MethodsA. Bidoret, E. Martins, B. Poncelet De Smet, and D. Poncelet4. Field Effect Microparticle Generation for Cell MicroencapsulationBrend Ray-Sea Hsu and Shin-Huei Fu5. Microfluidic Approach to Cell MicroencapsulationVarna Sharma, Michael Hunckler, Melur K. Ramasubramanian, Emmanuel C. Opara, and Kalyan  C. KaturiPart III Biomaterials and Enabling Technologies in Cell Microencapsulation6. Polymeric Materials for Cell MicroencapsulationA. Aijaz, D. Perera, and R. Olabisi<<7. Polymeric Materials for Perm-Selective Coating of Alginate MicrobeadsWilliam F. Kendall and Emmanuel C. Opara8. Determination of the Mechanical Strength of MicrocapsulesMarcus D. Darrabie and Emmanuel C. Opara9. The Diffusive Properties of Hydrogel Microcapsules for Cell EncapsulationD.M. Lavin, B.E. Bintz, and C.G. Thanos10. Methods for Incorporating Oxygen Generating Biomaterials into Cell Culture and Microcapsule SystemsJohn Patrick McQuilling and Emmanuel C. Opara11. Non-Invasive Tracking of Alginate-Microencapsulated CellsGenaro A. Paredes-Juarez, Brad P. Barnett, and Jeff W.M. Bulte12. Retrieval of Microencapsulated Islet Grafts for Post-Transplant EvaluationJohn Patrick McQuilling, Sivanandane Sittadjody, Rajesh Pareta, Samuel Pendergraft, Clancy J. Clark, Alan C. Farney, and Emmanuel C. OparaPart IV Isolation of Cells for Microencapsulation13. A Method of Porcine Pancreatic Islet Isolation for MicroencapsulationWilliam F. Kendall and Emmanuel C. Opara14: Selective Osmotic Shock (SOS)-Based Islet Isolation for MicroencapsulationKevin Enck, John P. McQuilling, Giuseppe Orlando, Riccardo Tamburrini, Sittadjody Sivanandane, and Emmanuel C. Opara15. Preparation and Characterization of Alginate–Chitosan Microcapsule for Hepatocyte CultureLamjuan Li, Yanhong Zhang, and Xiaoping Pan

 

16. Use of Flow Focusing Technique for Microencapsulation of Myoblasts J. Ciriza, L. Saenz del Burgo, RM Hernandez, G. Orive, and JL Pedraz

 

17. Alginate Microbeads for Cell and Protein Delivery Sami I. Somo, Omaditya Khanna, and Eric M. Brey

 

18. Compartmentalization of Two Cell Types in Multi-Layered Alginate Microcapsules Sivanandane Sittadjody, Justin M. Saul, and Emmanuel C. Opara19. Primary Choroid Plexus Tissue for Use in Cellular TherapyM.A. Sandrof and C.G. Thanos20. Microencapsulation of Stem Cells for TherapyShirae K. Leslie, Ramsey C. Kinney, Zvi Schwartz, and Barbara D. Boyan<21. Microencapsulated Cells for Cancer TherapyL. Saenz del Burgo, J. Ciriza, RM Hernández, G. Orive, and JL Pedraz22. Microencapsulation of Bacterial Cells by Emulsion Technique for Probiotic ApplicationSurajit Mandal and Subrota Hati