Buch, Englisch, Band 1232, 327 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 6395 g
Reihe: Methods in Molecular Biology
Buch, Englisch, Band 1232, 327 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 6395 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-5399-8
Verlag: Springer
This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico. Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties , and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Methods in Membrane Lipids, Second Edition seeks to aid scientist in further study into membrane lipids.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
1. Introduction to Membrane Lipids
Richard M. Epand
2. Introduction: Membrane properties (Good) for Life
Marek Cebecauer
3. Introduction To Fluorescence Probing Of Biological Membranes
Alexander P. Demchenko, Guy Duportail, Sule Oncul, Andrey S. Klymchenko, and Yves Mély
4. The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs)
Charles Cranfield, Sonia Carne, Boris Martinac, and Bruce Cornell
5. Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells
Deborah A. Brown
6. Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein Partitioning
KR Leventaland I Levental
7. Asymmetric Giant Lipid Vesicle Fabrication
Peichi C. Hu and Noah Malmstadt
8. Cholesterol Depletion using Methyl-b-cyclodextrin
Saleemulla Mahammad and Ingela Parmryd
9. A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systems
G. Ekin Atilla-Gokcumen and Ulrike S. Eggert
10. Imaging Membrane Order using Environmentally Sensitive Fluorophores
G Ashdown and DM Owen
11. 3D Super-resolution Imaging by Localisation Microscopy
Astrid Magenau and Katharina Gaus
12. Electron Microscopy Methods for Studying Plasma Membranes
Alison J. Beckett and Ian A. Prior
13. Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force Microscopy
Charles Roduit, Giovanni Longo, Giovanni Dietler, and Sandor Kasas
14. Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order
Richard KP Benninger
15. Scanning Fluorescence Correlation Spectroscopy on Biomembranes
Eduard Hermann, Jonas Ries, and Ana J. García-Sáez
16. X-ray Diffraction of Lipid Model Membranes
Arwen I.I. Tyler, Robert V. Law, and John M. Seddon
17. Solid State NMR of Lipid Model Membranes
Arwen I.I. Tyler, James A. Clarke, John M. Seddon, and Robert V. Law
18. Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis and Applications
Michael Carnell, Alex Macmillan, and Renee Whan
19. The Laurdan Spectral Phasor Method To Explore Membrane Micro-Heterogeneity And Lipid Domains In Live Cells
Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton
20. Cholesterol Behavior in Asymmetric Lipid Bilayers: Insights from Molecular Dynamics Simulations
Semen O. Yesylevskyy and Alexander P. Demchenko
21. Computer Simulations Of Phase Separation In Lipid Bilayers and Monolayers
Svetlana Baoukina and D. Peter Tieleman
