Pagano Cell Cycle - Materials and Methods

Erscheinungsjahr 2012
ISBN: 978-3-642-57783-3
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 287 Seiten, eBook

Reihe: Springer Lab Manual

During their lifetime, especially when growing and dividing, cells go through various steps of the cell cycle. Knowledge of the individual steps of the cell cycle will help us understand the development of a variety of diseases better, including cancer, and also to design new drugs against it. New techniques for studying the molecular basis of these processes have recently been developed and are described in detail in this manual. A glossary helps the reader to cope with the complex cell cycle terminology.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Pagano, Michele

Inhaltsverzeichnis

One Techniques for Studying the Mammalian Cell Cycle.- 1 Use of Immunohistochemistry in Cell Cycle Studies.- 2 Immunofluorescence Techniques in Cell Cycle Studies.- 3 Fluorescence in Situ Hybridization and Human Cell Cycle Genes.- 4 Techniques for Monitoring Cell Cycle Phases.- 5 Centrifugal Elutriation.- 6 Synchronization of Mammalian Cells.- 7 Microinjection and Electroporation of Macromolecules into Live Cells.- 8 Stable and Transient Transfections in Cell Cycle Studies.- 9 Quantification of Premature and Normal Mitosis.- Two Techniques for Studying the Xenopus Cell Cycle.- 10 Preparation of Xenopus Egg Extracts and Their Utilization in Cell Cycle Studies.- Three Techniques to Study the Yeast Cell Cycle.- 11 Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe.- 12 Preparation of Synchronous Cultures of the Fission yeast, Schizosaccharomyces Pombe.- Four Techniques for Studying the Cell Cycle of Any Species.- 13 Identification of Novel Cell Cycle Targets Using Small Molecule Ligands.- 14 Bacterial Expression and in Vitro Assay of Cell Cycle Kinases.- 15 Heterologous Expression and Secretion of Cell Cycle Proteins Using the Yeast Pichia pastoris.- 16 SV40 Based in Vitro DNA Replication Assay.- 17 From Sequence to Function-Structural Motifs of Cell Cycle Proteins.- 18 Purification of Cell Cycle Kinases.- 19 Interaction-Based Cloning of Novel Proteins.- 20 Expression of Cell Cycle Proteins Using Recombinant Baculoviruses.- 21 Cell Cycle-Regulated Transcription Factors: Bandshift Assays.- 22 Immunoprecipitation and Immunoblotting in Cell Cycle Studies.- 23 Protein mapping in Cell Cycle Studies.- 24 From Peptide to Purified Antibody.