E-Book, Englisch, Band 2314, 736 Seiten, eBook
Reihe: Methods in Molecular Biology
Parish / Kumar Mycobacteria Protocols
Fourth Auflage 2021
ISBN: 978-1-0716-1460-0
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
E-Book, Englisch, Band 2314, 736 Seiten, eBook
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-1460-0
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
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Weitere Infos & Material
Culturing Mycobacteria.- DNA Isolation from Mycobacteria.- Extraction and Separation of Mycobacterial Proteins.- Lipid and Lipoarabinomannan Isolation and Characterization.- Purification of Hibernating and Active C- Ribosomes from Zinc-Starved Mycobacteria.- Macrophage Infection Models for Mycobacterium tuberculosis .- Novel Single-Cell and High Throughput Microscopy Techniques to Monitor Dictyostelium discoideum - Mycobacterium marinum Infection Dynamics.- Single-Cell Analysis of Mycobacteria Using Microfluidics and Time-Lapse Microscopy.- Measuring Efflux and Permeability in Mycobacteria.- Drug Susceptibility Screening Using In Vitro Models of Hypoxic Non-Replicating Persistent Mycobacteria.- Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells.- Electroporation of Mycobacteria.- Gene Switching and Essentiality Testing.- Oligo-Mediated Recombineering and Its Use for Making SNPs, Knockouts, Insertions, and Fusions in Mycobacterium tuberculosis .- Using Proteolytic Hypomorphs to Detect Small Molecule Mechanism of Action.- CRISPR Interference (CRISPRi) for Targeted Gene Silencing in Mycobacteria.- Exploiting Fluorescent Proteins to Understand Mycobacterium tuberculosis Biology.- Metabolic Labeling of Live Mycobacteria with Trehalose-Based Probes.- Identification and Characterization of Mycobacterial Species Using Whole Genome Sequences.- Whole Genome Sequencing of Mycobacterium tuberculosis Directly from Sputum Samples.- Experimental and Computational Workflow for RNA Sequencing in Mycobacterium tuberculosis : From Total RNA to Differentially Expressed Genes.- RNA Sequencing for Transcript 5'-End Mapping in Mycobacteria.- Methods for Proteomic Analyses of Mycobacteria.- Targeted Lipidomics of Mycobacterial Lipids and Glycolipids.- Metabolomics of Mycobacterium tuberculosis .- Determining Minimum Inhibitory Concentrations in Liquid Cultures or on Solid Medium.- A Multi-Stress Model for High Throughput Screening Against Non-Replicating Mycobacterium tuberculosis .- Antimicrobial Susceptibility Testing for Mycobacterium sps in High Throughput Format.- High-Content Analysis Monitoring Intracellular Trafficking and Replication of Mycobacterium tuberculosis Inside Host Cells.- Efficient Measurement of Drug Interactions with DiaMOND (Diagonal Measurement of N-Way Drug Interactions).- Drug Sensitivity Testing of Mycobacterium tuberculosis Growing in a Hollow Fiber Bioreactor.