Pawlowski / Grelon / Armstrong Plant Meiosis

Methods and Protocols
2013
ISBN: 978-1-62703-333-6
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 238 Seiten, eBook

Reihe: Methods in Molecular Biology

Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation.  In plants, meiotic recombination is by far the most important source of genetic variation.  In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants.  These state-of -the-art protocols allow studying the organization and behavior of the genetic material in a wide range of both model and crop species.  Written in the highly successful Methods in Molecular Biology™series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Pawlowski, Wojciech P.

Grelon, Mathilde

Armstrong, Susan

Inhaltsverzeichnis

Section I: CYTOLOGICAL TECHNIQUES FOR LIGHT MICROSCOPY1. Spreading and Fluorescence in situ Hybridization (FISH) of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenenetical AnalysisSusan Armstrong 2. Analysis of Plant Meiotic Chromosomes by Chromosome PaintingMartin A. Lysak and Terezie Mandáková 3. Using Sequential Fluorescence and Genomic in situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napusElaine C. Howell and Susan Armstrong 4. Labeling Meiotic Chromosomes in Maize with Fluorescence in situ HybridizationZhi Gao, Fangpu Han, Tatiana V. Danilova, Jonathan C. Lamb, Patrice S. Albert, and James A. Birchler 5. Examining Female Meiocytes of Maize by Confocal MicroscopyPhilippa Barrell and Ueli Grossniklaus 6. Three-dimensional Acrylamide Fluorescence in situ Hybridization (FISH) for Plant CellsElizabeth S. Howe, Shaun P. Murphy, and Hank W. Bass 7. Analyzing Maize Meiotic Chromosomes with Super-resolution Structured Illumination MicroscopyChung-Ju Rachel Wang  8. Live Imaging of Chromosome Dynamics Moira J. Sheehan, R. Kelly Dawe, and Wojciech P. Pawlowski 9. Immunolocalization of Meiotic Proteins in Brassicacae – Method 1Liudmila A. Chelysheva, Laurie Grandont, Mathilde Grelon 10. Immunolocalization of Meiotic Proteins in Arabidopsis thaliana - Method 2Susan Armstrong and Kim Osman 11. Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana – Method 3Xiaohui Yang, Li Yuan and Christopher A, Makaroff 12. A Time Course for the Analysis of Meiotic Progression in Arabidopsis thalianaSusan Armstrong  13. Analyzing Meiotic Chromosomes in RiceZhukuan Cheng 14. Analysing Meiosis in BarleyJames D. Higgins Section II: CYTOLOGICAL TECHNIQUES FOR ELECTRON MICROSCOPY15. Preparing SC Spreads with RNs for EM AnalysisLorinda K. Anderson and Stephen M. Stack 16. Analysis of the Synaptonemal Complex in Brassica using TEMSusan Armstrong 17. Preparing Thin Sections of Meiotic Nuclei for Transmission Electron MicroscopyLjudmilla Timofejeva  Section III: GENETICS AND MOLECULAR BIOLOGY TECHNIQUES18. Characterization of Meiotic Non-crossover Molecules from Arabidopsis thaliana Pollen Hossein Khademian, Laurène Giraut, Jan Drouaud, and Christine Mézard 19. Chromatin Immunoprecipitation for Studying Chromosomal Localization of Meiotic Proteins in MaizeYan He, Gaganpreet Sidhu, and Wojciech P. Pawlowski 20. Analyzing the Meiotic Transcriptome using Isolated Meiocytes of Arabidopsis thalianaChangbin Chen and Ernest F. Retzel  21. Analysis of Meiotic Protein Complexes from Arabidopsis and Brassica using Affinity-based ProteomicsKim Osman, Elisabeth Roitinger, Jianhua Yang, Susan Armstrong, Karl Mechtler, and F. Chris H. Franklin 22. Identifying Meiotic Mutants in Arabidopsis thalianaWayne Crismani and Raphaël Mercier

Über Autor(innen)

Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation.  In plants, meiotic recombination is by far the most important source of genetic variation.  In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants.  These methods and protocols allows for understand the organization and behavior of the genetic material in a wide range of both model and crop species. extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.