Peeters Protides of the Biological Fluids

Proceedings of the Thirty-Third Colloquium, 1985
1. Auflage 2014
ISBN: 978-1-4832-7976-3
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 33, 722 Seiten, Web PDF

Reihe: Protides of the Biological Fluids

An International Review Series Devoted to Proteins and Related Studies, Volume 33: Protides of the Biological Fluids documents the proceedings of the 33rd Colloquium held in 1985. This book is divided into three sections. Section A focuses on the plasma protein genes that includes the location of genes on the chromosome, pathology and variance of several genes, and gene expression. The proteins of the nucleus and detection of antibodies, such as antinuclear antibodies (ANA), to nuclear constituents are deliberated in Section B. Section C is devoted to the technical advances in the field of protein chemistry. This book is a good reference for students, researchers, and medical practitioners conducting work on the protides of biological fluids.

Autoren/Hrsg.

Peeters, H.

Inhaltsverzeichnis

1;Front Cover;1
2;Protides of The Biological Fluids;4
3;Copyright Page;5
4;Table of Contents;6
5;PREFACE;22
6;ACKNOWLEDGEMENTS;24
7;Part 1: The Fourteenth Arne Tiselius MemorialLecture;26
7.1;CHAPTER 1. PROGRESS IN PLASMA PROTEINS:STRUCTURE, FUNCTION, AND GENES;28
7.1.1;I . INTRODUCTION: NEW DIRECTIONS IN PLASMA PROTEIN RESEARCH;28
7.1.2;I I . PRIMARY STRUCTURE OF THE PLASMA PROTEINS;28
7.1.3;I I I . THREE-DIMENSIONAL STRUCTURE AND ULTRASTRUCTURE OF PLASMA PROTEINS;30
7.1.4;IV. HEREDITARY DEFICIENCIES AND GENETIC POLYMORPHISM OF HUMAN PLASMA PROTEINS;31
7.1.5;V. CHROMOSOMAL MAPPING, GENOMIC ORGANIZATION, AND SITE-DIRECTED MUTAGENESIS OF HUMAN PLASMAPROTEINS;32
7.1.6;V I . INTERNAL DUPLICATIONS IN HUMAN PLASMA PROTEINS;33
7.1.7;ACKNOWLEDGEMENT;37
7.1.8;REFERENCES;37
8;SECTION A: Plasma Protein Genes;38
8.1;CHAPTER 2. ORGANIZATION AND CHROMOSOMALLOCATIONS OF GENES ENCODING HUMANPLASMA PROTEINS;40
8.1.1;ABSTRACT;40
8.1.2;KEY WORDS;40
8.1.3;INTRODUCTION;40
8.1.4;ACKNOWLEDGEMENT;45
8.1.5;REFERENCES;45
8.2;CHAPTER 3. SEQUENCE ANALYSIS OF HUMANIMMUNOGLOBULIN HEAVY-CHAIN GENES;46
8.2.1;ABSTRACT;46
8.2.2;KEYWORDS;46
8.2.3;HUMAN IMMUNOGLOBULIN HEAVY-CHAIN GENES;46
8.2.4;POTENTIAL Z-DNA FORMING AREA;47
8.2.5;SWITCH RECOMBINATION SEQUENCE INTRON EXTENDING FROM ym TO Cól.;48
8.2.6;REFERENCES;49
8.3;CHAPTER 4. STRUCTURE AND EVOLUTION OF THESERUM ALBUMIN GENE FAMILY;52
8.3.1;ABSTRACT;52
8.3.2;KEYWORDS;52
8.3.3;INTRODUCTION;52
8.3.4;THE MOLECULAR CLOCK;53
8.3.5;PERIODIC DOMAINS IN THE ALBUMIN AND AFP GENES;54
8.3.6;INVASION OF THE ALBUMIN GENE BY ALU REPEATS;55
8.3.7;ACKNOWLEDGEMENT;55
8.3.8;REFERENCES;55
8.4;CHAPTER 5. MOLECULAR GENETICS OF HUMANTRANSFERRIN;56
8.4.1;ABSTRACT;56
8.4.2;KEY WORDS;56
8.4.3;INTRODUCTION;56
8.4.4;MATERIALS AND METHODS;57
8.4.5;RESULTS AND DISCUSSION;57
8.4.6;ACKNOWLEDGEMENT;59
8.4.7;REFERENCES;59
8.5;CHAPTER 6. APOLIPOPROTEIN A-II GENE STRUCTURE:EVOLUTIONARY IMPLICATIONS;60
8.5.1;ABSTRACT;60
8.5.2;KEY WORDS;60
8.5.3;INTRODUCTION;60
8.5.4;EXPERIMENTAL PROCEDURES;60
8.5.5;RESULTS AND DISCUSSION;61
8.5.6;ACKNOWLEDGMENT;63
8.5.7;REFERENCES;63
8.6;CHAPTER 7. MOLECULAR GENETICS OF HAPTOGLOBIN;64
8.6.1;ABSTRACT;64
8.6.2;KEYWORDS;64
8.6.3;INTRODUCTION;64
8.6.4;40 GENE STRUCTUREMATERIALS AND METHODS;65
8.6.5;RESULTS;65
8.6.6;ACKNOWLEDGMENTS;66
8.6.7;REFERENCES;67
8.7;CHAPTER 8. HUMAN COMPLEMENT GENES OF THEMAJOR HISTOCOMPATIBILITY COMPLEX;68
8.7.1;ABSTRACT;64
8.7.2;KEYWORDS;64
8.7.3;INTRODUCTION;64
8.7.4;MATERIALS AND METHODS;65
8.7.5;RESULTS;65
8.7.6;ACKNOWLEDGMENTS;66
8.7.7;REFERENCES;67
8.8;CHAPTER 9. HUMAN COMPLEMENT GENES OF THEMAJOR HISTOCOMPATIBILITY COMPLEX;68
8.8.1;ABSTRACT;68
8.8.2;KEYWORDS;68
8.8.3;INTRODUCTION;68
8.8.4;MAPPING THE HLA CLASS III LOCI;69
8.8.5;THE FACTOR B GENE STRUCTURE;69
8.8.6;HLA CLASS III GENETIC POLYMORPHISM;70
8.8.7;REFERENCES;70
8.9;CHAPTER 10. COAGULATION AND FIBRINOLYSIS:CHARACTERIZATION OF THE HUMANPROTHROMBIN AND HUMAN TISSUEPLASMINOGENACTIVATOR GENES;72
8.9.1;ABSTRACT;72
8.9.2;KEYWORDS;72
8.9.3;INTRODUCTION;72
8.9.4;RESULTS AND DISCUSSION;73
8.9.5;REFERENCES;74
8.10;CHAPTER 11. THE PRESENCE OF MULTIPLEPOLYADENYLATION SITES IN THE GENEFOR THE â AND 7 CHAINS OF HUMANFIBRINOGEN;76
8.10.1;ABSTRACT;76
8.10.2;KEYWORDS;76
8.10.3;INTRODUCTION;76
8.10.4;CLONING OF .-FIBRINOGEN cDNA AND GENE;77
8.10.5;CLONING OF fijFIBRINOGEN, cDNA AND.GEffE;78
8.10.6;DISCUSSION;78
8.10.7;ACKNOWLEDGMENT;78
8.10.8;REFERENCES;78
8.11;CHAPTER 12. THE HUMAN FACTOR VIII PROTEIN;80
8.11.1;INTRODUCTION;80
8.11.2;STRUCTURE OF FACTOR VIII;80
8.11.3;HOMOLOGY OF FACTOR VIII AND PLASTOCYANIN;81
8.11.4;PREDICTION OF SECONDARY STRUCTURE AND HYDROPATHY OF FACTOR VIII;82
8.11.5;DISCUSSION;83
8.11.6;REFERENCES;83
8.12;CHAPTER 13. STRUCTURE OF THE GENE FOR HUMANFACTOR IX;84
8.12.1;ABSTRACT;84
8.12.2;KEYWORDS;84
8.12.3;INTRODUCTION;84
8.12.4;STRUCTURE OF THE GENE FOR HUNAN FACTOR IX;84
8.12.5;ACKNOWLEDGMENT;87
8.12.6;REFERENCES;87
8.13;CHAPTER 14. STRUCTURE AND EVOLUTION OF THEHUMAN ANTITHROMBIN III GENE;88
8.13.1;Abstract;88
8.13.2;Introduction;88
8.13.3;Expérimental Procedures;88
8.13.4;Results and Discussion;89
8.13.5;ACKNOWLEDGEMENTS;90
8.13.6;REFERENCES;90
8.14;CHAPTER 15. THE STRUCTURE OF THE HUMANPLASMINOGEN ACTIVATOR (uPA) GENE;92
8.14.1;Abstract;92
8.14.2;Introduction.;92
8.14.3;Methods.;92
8.14.4;Results;92
8.14.5;Conclusions;94
8.14.6;Acknowledgements;94
8.14.7;References;94
8.15;CHAPTER 16. CHROMOSOME MAPPING OF SERUMPROTEIN GENES;96
8.15.1;ABSTRACT;96
8.15.2;KEY WORDS;96
8.15.3;INTRODUCTION;96
8.15.4;MATERIALS AND METHODS;97
8.15.5;RESULTS;98
8.15.6;DISCUSSION;99
8.15.7;ACKNOWLEDGEMENT;101
8.15.8;REFERENCES;101
8.16;CHAPTER 17. CLONING AND EXPRESSION IN MICROORGANISMSOF THE GENETICINFORMATION CODING FOR HUMANPLASMA PROTEINS;102
8.16.1;1· Introduction;102
8.16.2;2. Od -anti trypsin;103
8.16.3;3. Antithrombin III;104
8.16.4;4· Haptoglobin;105
8.16.5;5. Uroki nase;105
8.16.6;6. Conclusion;106
8.16.7;References;107
8.17;CHAPTER 18. IN SITU HYBRIDIZATION FORCHROMOSOMAL MAPPING OF PLASMAPROTEINS;108
8.17.1;ABSTRACT;108
8.17.2;KEY WORDS;108
8.17.3;INTRODUCTION;108
8.17.4;MATERIALS AND METHODS;109
8.17.5;RESULTS AND DISCUSSION;110
8.17.6;ACKNOWLEDGEMENT;111
8.17.7;REFERENCES;111
8.18;CHAPTER 19. IN SITU HYBRIDIZATION: LOCATION OFPLASMA PROTEIN TRANSCRIPTION;112
8.18.1;ABSTRACT;112
8.18.2;KEY WORDS;112
8.18.3;INTRODUCTION;112
8.18.4;RESULTS AND DISCUSSION;114
8.18.5;ACKNOWLEDGEMENT;115
8.18.6;REFERENCES;115
8.19;CHAPTER 20. STRUCTURE AND LIVER SPECIFICEXPRESSION OF THE HUMAN RETINOLBINDING PROTEIN GENE;116
8.19.1;ABSTRACT;116
8.19.2;KEYWORDS;116
8.19.3;INTRODUCTION;116
8.19.4;RESULTS AND DISCUSSION;116
8.19.5;REFERENCES;119
8.20;CHAPTER 21. ORGANIZATION OF THE GENES CODINGFOR PROTHROMBIN AND FACTOR X;120
8.20.1;ABSTRACT;120
8.20.2;KEYWORDS;120
8.20.3;INTRODUCTION;120
8.20.4;PROTHROMBIN;121
8.20.5;FACTOR X;121
8.20.6;ACKNOWLEDGEMENTS;122
8.20.7;REFERENCES;123
8.21;CHAPTER 22. MAPPING TISSUE PLASMINOGENACTIVATOR AND URO KIN AS E GENES TOHUMAN CHROMOSOMES;124
8.21.1;ABSTRACT;124
8.21.2;KEYWORDS;124
8.21.3;INTRODUCTION;124
8.21.4;RESULTS AND DISCUSSION;124
8.21.5;ACKNOWLEDGEMENTS;125
8.21.6;REFERENCES;126
8.22;CHAPTER 23. MOLECULAR GENETICS OF HUMANANTITHROMBIN III DEFICIENCIES;128
8.22.1;ABSTRACT;128
8.22.2;INTRODUCTION;128
8.22.3;MATERIALS AND METHODS;128
8.22.4;RESULTS AND DISCUSSION;128
8.22.5;SUMMARY;131
8.22.6;ACKNOWLEDGEMENTS;131
8.22.7;REFERENCES;131
8.23;CHAPTER 24. GENETICALLY ABNORMAL HUMANFIBRINOGENS — A SUMMARY OF 32STRUCTURALLY ELUCIDATED VARIANTS;132
8.23.1;ABSTRACT;132
8.23.2;KEYWORDS;132
8.23.3;INTRODUCTION;132
8.23.4;RESULTS AND DISCUSSION;133
8.23.5;CONCLUSION;135
8.23.6;REFERENCES;135
8.24;CHAPTER 25. LINKAGE OF HUMAN APOLIPOPROTEINAI, CHI AND AIV GENES;136
8.24.1;ABSTRACT;136
8.24.2;KEY WORDS;136
8.24.3;INTRODUCTION;136
8.24.4;MATERIALS AND METHODS;137
8.24.5;RESULTS AND DISCUSSION;137
8.24.6;ACKNOWLEDGEMENT;139
8.24.7;REFERENCES;139
8.25;CHAPTER 26. GENETIC POLYMORPHISMS AND LINKAGERELATIONS OF COMPLEMENT FACTOR C8;140
8.25.1;ABSTRACT;140
8.25.2;KEYWORDS;140
8.25.3;INTRODUCTION;140
8.25.4;MATERIALS AND METHODS;140
8.25.5;RESULTS AND DISCUSSION;140
8.25.6;REFERENCES;142
8.26;CHAPTER 27. CLOSE GENETIC LINKAGE BETWEENFOUR PLASMA a-PROTEASE INHIBITORLOCI (Pil, Pol A, PolB, Pi2) IN PIGS;144
8.26.1;ABSTRACT;144
8.26.2;KEYWORDS;144
8.26.3;INTRODUCTION;144
8.26.4;MATERIALS AND METHODS;144
8.26.5;RESULTS AND DISCUSSION;145
8.26.6;ACKNOWLEDGEMENT;147
8.26.7;REFERENCES;147
8.27;CHAPTER 28. GENOMIC EVIDENCE FOR THE TRULYALLELIC NATURE OF THE B-LOCUSPOLYMORPHISM OF THE RABBITIMMUNOGLOBULIN LIGHT CHAIN;148
8.27.1;ABSTRACT;148
8.27.2;INTRODUCTION;148
8.27.3;RESULTS;148
8.27.4;DISCUSSION.;150
8.27.5;ACKNOWLEDGMENTS;151
8.27.6;REFERENCES;151
8.28;CHAPTER 29. GENETIC STUDIES ON A HUMAN PLASMATRANSTHYRETIN (PREALBUMIN) VARIANTASSOCIATED WITH FAMILIALAMYLOIDOTIC POLYNEUROPATHY;152
8.28.1;ABSTRACT;152
8.28.2;KEYWORDS;152
8.28.3;INTRODUCTION;152
8.28.4;MATERIALS AND METHODS;153
8.28.5;RESULTS AND DISCUSSION;153
8.28.6;REFERENCES;155
8.29;CHAPTER 30. GENE ORGANIZATION AND DNAPOLYMORPHISM OF THE HUMANTRANSFERRIN GENE;156
8.29.1;ABSTRACT;156
8.29.2;INTRODUCTION;156
8.29.3;MATERIAL AND METHODS;156
8.29.4;RESULTS AND DISCUSSION;157
8.29.5;REFERENCES;159
8.30;CHAPTER 31. EXPRESSION IN E. coli OF HUMANPREPROUROKINASE cDNA;160
8.30.1;ABSTRACT;160
8.30.2;INTRODUCTION;160
8.30.3;MATERIALS AND METHODS;160
8.30.4;RESULTS;161
8.30.5;DISCUSSION;163
8.30.6;REFERENCES;163
8.31;CHAPTER 32. CELL CYCLE DEPENDENT VARIATIONS INTHE DISTRIBUTION OF THE NUCLEARPROTEIN CYCLIN (PCNA) IN CULTUREDCELLS;164
8.31.1;INTRODUCTION;164
8.31.2;CONCLUSIONS;167
8.31.3;ACKNOWLEDGEMENTS;167
8.31.4;REFERENCES;167
8.32;CHAPTER 33. ORGANIZATION AND EVOLUTION OF THEMOUSE COMPLEMENT C4 ISOTYPES;168
8.32.1;ABSTRACT;168
8.32.2;KEYWORDS;168
8.32.3;REFERENCES;171
8.33;CHAPTER 34. ALTERED INHIBITION PROPERTIES OFarANTITRYPSIN VARIANTS CONSTRUCTEDBY SITE-DIRECTED MUTAGENESIS;172
8.33.1;ABSTRACT;172
8.33.2;KEYWORDS;172
8.33.3;INTRODUCTION;172
8.33.4;PRODUCTION OF aG.. IN E. COLI;173
8.33.5;arAT AND CIGARETTE SMOKING;173
8.33.6;OXIDATION RESISTANT arAT;173
8.33.7;AN arAT VARIANT THAT INHIBITS THROMBIN;174
8.34;CHAPTER 35. STRUCTURE OF THE HUMANHAPTOGLOBIN PSEUDOGENE;176
8.34.1;Abstract;176
8.34.2;Introduction;176
8.34.3;Results and discussion;176
8.34.4;References;180
8.35;CHAPTER 36. MOLECULAR CLONING, SEQUENCING ANDEXPRESSION OF A cDNA CODING FORHUMAN ANTITHROMBIN III;182
8.35.1;ABSTRACT;182
8.35.2;References;184
8.36;CHAPTER 37. PURIFICATION OF ACTIVE HUMANaj-ANTITRYPSIN FROM GENETICALLYENGINEERED MICROORGANISMS;186
8.36.1;ABSTRACT;186
8.36.2;KEYWORDS;186
8.36.3;INTRODUCTION;186
8.36.4;MATERIALS AND METHODS;186
8.36.5;RESULTS;187
8.36.6;DISCUSSION;188
8.36.7;REFERENCES;189
8.37;CHAPTER 38. REGULATION OF RAT a2-MACROGLOBULIN GENE ACTIVITY;190
8.37.1;(^-Macroglobulin Gene lranscription in Isolated Nuclei;190
8.37.2;Factors Involved in Rat otg-Macroglobul in Gene Activation;190
8.37.3;Isolation of the Rat a 2 ~ . . ( : G 091...1 in Gene;190
8.37.4;References;190
8.38;CHAPTER 39. INFLUENCE OF NEONATALANDROGENIZATION ON THE EXPRESSIONOF GL2u-GLOBULIN GENES;194
8.38.1;ABSTRACT;194
8.38.2;KEYWORDS;194
8.38.3;INTRODUCTION;194
8.38.4;MATERIALS AND METHODS;194
8.38.5;RESULTS;195
8.38.6;DISCUSSION;196
8.38.7;ACKNOWLEDGEMENTS;197
8.38.8;REFERENCES;197
8.39;CHAPTER 40. PREFERENTIAL SEGREGATION OF MURINECHROMOSOME 11 IN TCGF-INDEPENDENTRAT x MOUSE T-CELL HYBRIDS;198
8.39.1;ABSTRACT;198
8.39.2;KEYWORDS;198
8.39.3;INTRODUCTION;198
8.39.4;MATERIALS AND METHODS;198
8.39.5;RESULTS AND DISCUSSION;199
8.39.6;ACKNOLEDGEMENT;201
8.39.7;REFERENCES;201
8.40;CHAPTER 41. INDIVIDUAL POLYMORPHISM OFALBUMIN GENE STUDIED BYSEQUENCING DNA;202
8.40.1;ABSTRACT;202
8.40.2;KEYWORDS;202
8.40.3;INTRODUCTION;202
8.40.4;RESULTS;202
8.40.5;REFERENCES;204
8.40.6;AOENOWUÎDGEMENT;204
9;SECTION B: Antinudear Antibodies (ANA);206
9.1;CHAPTER 42. FINE STRUCTURE OF snRNPs;208
9.1.1;ABSTRACT;208
9.1.2;KEYWORDS;208
9.1.3;INTRODUCTION;208
9.1.4;THE CORE snRNP CONCEPT;208
9.1.5;TOWARDS NATIVE snRNPs;209
9.1.6;REFERENCES;210
9.2;CHAPTER 43. ANTI-Sm AND ANTI-La MURINEMONOCLONAL ANTIBODIES:CHARACTERISATION OF THE ANTIGENS;212
9.2.1;ABSTRACT;212
9.2.2;KEYWORDS;212
9.2.3;INTRODUCTION;212
9.2.4;RESULTS;213
9.2.5;Acknowledgements;215
9.2.6;References;215
9.3;CHAPTER 44. ANTIHISTONE ANTIBODIES BIND TORESTRICTED EPITOPES;216
9.3.1;ABSTRACT;216
9.3.2;KEY WORDS;216
9.3.3;INTRODUCTION;216
9.3.4;MATERIALS AND METHODS;216
9.3.5;RESULTS;217
9.3.6;REACTIVITY OF SLE AND DIL ANTIBODIES WITH H2A PEPTIDES;217
9.3.7;REFERENCES;219
9.4;CHAPTER 45. IP-HETEROGENEITY OF NUCLEARANTIGENS DETECTED IN AN ISOELECTRICFOCUSING-IMMUNOBLOT SYSTEM;220
9.4.1;ABSTRACT;220
9.4.2;KEYWORDS;220
9.4.3;INTRODUCTION;220
9.4.4;MATERIALS AND METHODS;220
9.4.5;RESULTS;221
9.4.6;DISCUSSION;223
9.4.7;M