Peeters Separation of Cells and Subcellular Elements

Proceedings of a Meeting Organised by EFRAC (European Working Party for the Separation and Detection of Biological Fractions), Sponsored by the Committee for Science and Technology of the Council of Europe, Brussels, 4-5 May 1979
1. Auflage 2017
ISBN: 978-1-4831-8935-2
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 116 Seiten, Web PDF

Separation of Cells and Subcellular Elements presents the chemical engineering approaches in bio-separation methods. This book evaluates the cellular aggregation in turbulent suspension, the phase partition of cells and subcellular particles, and the continuous free-flow electrophoresis. The first chapters deal with flow cytometric characterization of tumor associated changes in gynecologic malignancies; steady state rheolectrolysis; and electrophoretic approaches applicable to cell separation. The succeeding chapters consider the chromosome separation by velocity sedimentation at unit gravity and the separation of human lymphocytes based on volume and density. Other chapters describe the analysis of the gradient former for zonal rotors and the centrifugal elutriation of living cells. The last chapters are devoted to the human platelet isolation from whole blood on metrizamide gradients. The book can provide useful information to biochemists, cytologists, students, and researchers.

Autoren/Hrsg.

Peeters, H.

Inhaltsverzeichnis

1;Front Cover;1
2;Separation of Cells and Subcellular Elements;4
3;Copyright Page ;5
4;Table of Contents;6
5;PREFACE;9
6;PART I: GENERAL PROBLEMS;10
6.1;CHAPTER 1. CHEMICAL ENGINEERING APPROACHES IN BIO-SEPARATION METHODS;10
6.1.1;ABSTRACT;10
6.1.2;KEYWORDS;10
6.1.3;INTRODUCTION;10
6.1.4;COLUMN DESIGN CONSIDERATIONS;11
6.1.5;CHEMICAL ENGINEERING CONCEPTS;13
6.1.6;CONCLUSIONS;15
6.1.7;REFERENCES;15
6.2;CHAPTER 2. CELLULAR AGGREGATION IN TURBULENT SUSPENSIONS;16
6.2.1;ABSTRACT;16
6.2.2;INTRODUCTION;16
6.2.3;THEORY;17
6.2.4;EXPERIMENTAL RESULTS AND DISCUSSION;18
7;PART II: FLOW CYTOMETRY;20
7.1;CHAPTER 3. FLOW CYTOMETRIC CHARACTERISATION OF TUMOR ASSOCIATED CHANGES IN GYNECOLOGIC MALIGNANCIES;20
7.1.1;Abstract;20
7.1.2;Keywords;20
7.1.3;1. Introduction;20
7.1.4;2. Materials and Methods;21
7.1.5;3. Results;22
7.1.6;4. Discussion;24
7.1.7;Acknowledgement;25
7.1.8;5. References;25
8;PART III: PHASE PARTITION;26
8.1;CHAPTER 4. PHASE PARTITION OF CELLS AND SUBCELLULAR PARTICLES;26
8.1.1;ABSTRACT;26
8.1.2;KEYWORDS;26
8.1.3;INTRODUCTION;26
8.1.4;APPLICATION;28
8.1.5;CONCLUSION;30
8.1.6;REFERENCES;31
9;PART IV: ELECTROPHORESIS;32
9.1;CHAPTER 5. CONTINUOUS FREE-FLOW ELECTROPHORESIS;32
9.1.1;ABSTRACT;32
9.1.2;PRINCIPLE;32
9.1.3;THE INFLUENCE OF MECHANICAL AND ELECTROKINETIC VARIABLES ON THE EFFICIENCY OF THE METHOD;32
9.1.4;AN ANALYTICAL VERSION OF FREE-FLOW ELECTROPHORESIS;36
9.1.5;REFERENCES;39
9.2;CHAPTER 6. NEW ELECTROPHORETIC APPROACHES APPLICABLE TO CELL SEPARATION;40
9.2.1;ABSTRACT;40
9.2.2;INTRODUCTION;41
9.2.3;CELL SIEVING;41
9.2.4;CELL ELECTROFOCUSING;43
9.2.5;REFERENCES;44
9.3;CHAPTER 7. STEADY STATE RHEOELECTROLYSIS. II. EXPERIMENTAL ENDEAVOUR;48
9.3.1;ABSTRACT;48
9.3.2;KEYWORDS;48
9.3.3;INTRODUCTION;48
9.3.4;EXPERIMENTAL;49
9.3.5;DISCUSSION;53
9.3.6;ACKNOWLEDGEMENT;53
9.3.7;REFERENCES;53
10;PART V: UNIT GRAVITY SEDIMENTATION;54
10.1;CHAPTER 8. A SORTER OF CELLS, CHROMOSOMES AND NUCLEI THAT COMBINES SIMPLICITY WITH GOOD RESOLUTION;54
10.1.1;ABSTRACT;54
10.1.2;INTRODUCTION;54
10.1.3;MATERIALS AND METHODS;54
10.1.4;RESULTS;56
10.1.5;ACKNOWLEDGEMENT;59
10.1.6;REFERENCES;59
10.2;CHAPTER 9. CHROMOSOME SEPARATION BY VELOCITY SEDIMENTATION AT UNIT GRAVITY;60
10.2.1;ABSTRACT;60
10.2.2;INTRODUCTION;60
10.2.3;MATERIALS AND METHODS;60
10.2.4;RESULTS AND DISCUSSION;62
10.2.5;REFERENCES;63
11;PART VI: ULTRACENTRIFUGAL METHODS;64
11.1;CHAPTER 10. CENTRIFUGAL ELUTRIATION: SEPARATION OF LIVING CELLS;64
11.1.1;ABSTRACT;64
11.1.2;KEYWORDS;64
11.1.3;INTRODUCTION;64
11.1.4;PRINCIPLES OF CENTRIFUGAL ELUTRIATION;65
11.1.5;ADVANTAGES OF THE SYSTEM;66
11.1.6;APPLICATIONS;66
11.1.7;REFERENCES;67
11.2;CHAPTER 11. A NEW GRADIENT FORMER FOR ZONAL ROTORS;70
11.2.1;ABSTRACT;70
11.2.2;KEY WORDS;70
11.2.3;INTRODUCTION;70
11.2.4;DESIGN OF GRADIENTFORMER;71
11.2.5;THEORETICAL CONSIDERATIONS;71
11.2.6;MATERIALS AND METHODS;73
11.2.7;RESULTS AND DISCUSSION;73
11.2.8;REFERENCES;75
11.3;CHAPTER 12. THE USE OF DENSITY GRADIENTS OF PER COLL® FOR THE SEPARATION OF BIOLOGICAL PARTICLES;76
11.3.1;ABSTRACT;76
11.3.2;KEYWORDS;76
11.3.3;INTRODUCTION;76
11.3.4;PHYSICAL PROPERTIES OF PERCOLL;77
11.3.5;NEW APPLICATIONS FOR PERCOLL;77
11.3.6;BUOYANT DENSITIES OF BIOLOGICAL PARTICLES IN PERCOLL;79
11.3.7;ACKNOWLEDGEMENT;81
11.3.8;REFERENCES;81
11.4;CHAPTER 13. IMPROVED SEPARATION OF HUMAN PERIPHERAL T-CELLS USING PVP-COATED COLLOIDAL SILICA PARTICLES (PERCOLL);82
11.4.1;ABSTRACT;82
11.4.2;INTRODUCTION;82
11.4.3;MATERIALS AND METHODS;83
11.4.4;RESULTS;84
11.4.5;DISCUSSION;85
11.4.6;REFERENCES;85
11.5;CHAPTER 14. SEPARATION OF HUMAN LYMPHOCYTES ON THE BASIS OF VOLUME AND DENSITY;86
11.5.1;ABSTRACT;86
11.5.2;INTRODUCTION;86
11.5.3;MATERIALS AND METHODS;86
11.5.4;RESULTS;87
11.5.5;CONCLUSIONS;90
11.5.6;REFERENCES;91
11.6;CHAPTER 15. HUMAN PLATELET ISOLATION FROM WHOLE BLOOD ON METRIZAMIDE GRADIENTS;92
11.6.1;ABSTRACT;92
11.6.2;KEY-WORDS;92
11.6.3;INTRODUCTION;92
11.6.4;IN SUMMARY;95
11.6.5;REFERENCES;95
11.7;CHAPTER 16. ISOLATION OF DENSE BODIES FROM HUMAN BLOOD PLATELETS USING DISCONTINUOUS METRIZAMIDE GRADIENTS;96
11.7.1;ABSTRACT;96
11.7.2;KEY-WORDS;96
11.7.3;INTRODUCTION;96
11.7.4;ISOLATION OF DENSE BODIES;96
11.7.5;CHARACTERIZATION OF THE DENSE BODIES;98
11.7.6;CONCLUSIONS;99
11.7.7;REFERENCES;99
11.8;CHAPTER 17. EXCHANGE OF CHOLESTEROL BETWEEN THE SUBCELLULAR FRACTIONS DURING DIFFERENTIAL CENTRIFUGATION OF RAT ADRENOCORTICAL TISSUE;100
11.8.1;ABSTRACT;100
11.8.2;KEYWORDS;100
11.8.3;INTRODUCTION;100
11.8.4;MATERIAL AND METHODS;101
11.8.5;RESULTS AND COMMENTS;101
11.8.6;ACKNOWLEDGEMENT;102
11.8.7;REFERENCES;102
11.9;CHAPTER 18. THE ISOLATION OF STABLE CATTLE ROD OUTER SEGMENTS WITH AN INTACT PLASMA MEMBRANE;104
11.9.1;ABSTRACT;104
11.9.2;KEYWORDS;104
11.9.3;INTRODUCTION;104
11.9.4;PROCEDURES;104
11.9.5;RESULTS;105
11.9.6;DISCUSSION;107
11.9.7;ACKNOWLEDGEMENT;107
11.9.8;REFERENCES;107
11.10;CHAPTER 19. STUDIES OF LARGE SCALE PREPARATION OF POLYMERIZED HUMAN RED CELLS GHOSTS ABLE TO NEUTRALIZE BLOOD GROUPS ANTIBODIES;108
11.10.1;ABSTRACT;108
11.10.2;INTRODUCTION;108
11.10.3;MATERIALS AND METHODS;108
11.10.4;RESULTS;109
11.10.5;DISCUSSION;111
11.10.6;References;111
11.11;CHAPTER 20. LARGE-SCALE CONCENTRATION AND PURIFICATION OF VARIOUS ANIMAL VIRUSES USING ISOPYCNIC BANDING IN A CONTINUOUS-FLOW ULTRA-CENTRIFUGE;112
11.11.1;ABSTRACT;112
11.11.2;KEYWORDS;112
11.11.3;REFERENCES;115
12;INDEX;116