Peng / Lim Biomolecular Display Technologies

Erscheinungsjahr 2026
ISBN: 978-1-0716-5249-7
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 316 Seiten

Reihe: Methods in Molecular Biology

This volume explores major biomolecular display platforms and their applications, which allow researchers to mine vast, diverse libraries for functional variants, bridging molecular design and real-world application. Beginning with a section on foundational techniques for functional libraries, the book continues by covering tailored yeast, phage, and cDNA display strategies to boost efficiency, modulate binding, and engineer scaffolds for specialized needs, display-driven identification of macrocyclic ligands, nanoparticle-binding peptides, epitopes, and functional proteins, as well as computational advances, protein engineering, and post-selection analysis to refine hits and accelerate translation. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, offers expert guidance and insight into the transformative potential of display technologies.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Peng, Huan

Lim, Theam Soon

Inhaltsverzeichnis

Incorporation of Butyryl-Lysine into Phage-Displayed Peptide Libraries.- Construction and Functional Validation of a High-Diversity Naive Phage Antibody Library.- Construction of a Cyclic Peptide Library for Phage Display.- Generation of a CDR H3 Randomized Monoclonal Antibody Library by Kunkel Mutagenesis.- Yeast Display of Single-Chain Fc Fragments with Modulated Binding of Effector Molecules.- A Simple and Rapid Method for Preparing VHH Antibody-Template DNA Conjugates for Loop-Mediated Isothermal Amplification (LAMP) Method from cDNA Display.- M13 Bacteriophage pVIII Protein: Display Strategies, Chemical Modification, and Antibacterial Activity Investigations.- Yeast Molecular Display for the Screening and Production of Immunological Proteins Using Nanobody as an Example.- Application of Phage-Displayed Peptide Library for Epitope Mapping.- Phage Display Biopanning Strategy for Discovery of Nanoparticle-Binding Peptides.- De Novo Discovery of Prenylated Macrocyclic Peptide Ligands.- Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.- AI-Driven Protein Research: From Prediction to Design.- Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.- High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.- Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.- Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.