Piepenbrink / Auchtung Clostridioides difficile
Erscheinungsjahr 2026
ISBN: 978-1-0716-5328-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
Methods and Protocols
E-Book, Englisch, 244 Seiten
Reihe: Springer Protocols
ISBN: 978-1-0716-5328-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
This volume discusses the latest advancements on approaches used to characterize
physiology, including new methods for genetic modifications and novel models for studying host-pathogen interactions. The chapters in this book cover various topics such as FASTQ; CRISPR-AsCas12a and dAsCas 12a-mediated gene knockout and knockdown in
preparation of spores with 70:30 method; measuring
adhesion to mucosal surfaces; and isolating
from animal samples. Written in the highly successful
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical,
is a valuable resource for researchers interested in learning more about this field. Gaining a deeper understanding of
physiology is crucial for developing new therapies for treatment and predicting the future evolution of this important clinical pathogen.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
From FASTQ to Function to Phylogeny: Methods for Antimicrobial Resistance and Outbreak Investigation.- Generating Targeted Mutants Using a Riboswitch to Control Plasmid Behavior During Allelic Exchange Mutagenesis.- Use of the Golden Gate Cloning Technique for Plasmid Construction to Generate Conditional Lethal Mutants in .- CRISPR-AsCas12a and dAsCas 12a-Mediated Gene Knockout and Knockdown in Isolation and Characterization of Bacteriophages.- Preparation of Spores with 70:30 Method.- An Integrated Workflow for the Purification and Fluorescence Imaging of Spores Using Lanthanide Beta-Diketone Complexes.- Nanoluciferase-Based Reporters for Working with in a Hypoxia Chamber.- Dual Fluorescent Labeling for Bacterial Aggregation Assays.- In Vitro Measurement of Biofilm Formation Induced by Gut and Microbiota-Derived Signals.- Measuring Adhesion to Mucosal Surfaces.- An In Vitro Model for Studying Interactions between Gastrointestinal Microbes and Planktonic and Sessile Populations.- Conventional, Gnotobiotic, and Humanized Microbiota Mouse Models of Infection.- Visualizing During Murine Infection.- Infection in Animals.- Isolating from Animal Samples.
