Polizzi / Kontoravdi Synthetic Biology

Erscheinungsjahr 2013
ISBN: 978-1-62703-625-2
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1073, 226 Seiten, eBook

Reihe: Methods in Molecular Biology

In Synthetic Biology , expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology.  Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Polizzi, Karen M.

Kontoravdi, Cleo

Inhaltsverzeichnis

What is Synthetic Biology?.- Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program.- BioBrick ™ Assembly Using the In-Fusion PCR Cloning Kit.- Overlap Extension PCR Cloning.- One-Step Isothermal Assembly of DNA Fragments.- Creation and Characterization of Component Libraries for Synthetic Biology.- In vivo and in vitro Characterisation of s 70 Constitutive Promoter BioParts by Real-time PCR and Fluorescent Measurements.- In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli.- Construction and Engineering of  Large Biochemical Pathways via DNA Assembler.- Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick Vectors.- Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research.- Combinatorial DNA Assembly using Golden Gate Cloning.- Construction of Synthetic Gene Circuits in the Escherichia coli Genome.- Shuffling of DNA Cassettes in a Synthetic Integrin.- Systematic Methodology for the Development of Mathematical Models for Biological Processes.-SOBOLHDMR: A General Purpose Modelling Software.