Popescu Ionotropic Glutamate Receptor Technologies

1. Auflage 2016
ISBN: 978-1-4939-2812-5
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 106, 304 Seiten, eBook

Reihe: Neuromethods

This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology.  Chapters in this book cover methods to help illuminate the assembly, trafficking, molecular composition and subcellular location of iGluRs; approaches used to understand the atomic organization of iGluRs; and techniques to monitor receptor activity in real time. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. Concise and easy-to-use, Ionotropic Glutamate Receptor Technologies aims to facilitate the implementation of specific methods to iGluR investigations.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Popescu, Gabriela K.

Inhaltsverzeichnis

Assaying AMPA Receptor Oligomerization.- A Step-by-Step Guide to Single Subunit Counting Of Membrane-Bound Proteins in Mammalian Cells.- Counting NMDA Receptors at the Cell Surface.- Electron Microscopy Analysis of AMPA receptors in Dendritic Spines.- Electrophysiological Tagging of Ionotropic Receptors.- Functional Detection of Novel Triheteromeric NMDA Receptors.- Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses.- NMR Approaches to Functional Dynamics Of Genetically Separated iGluR Domains.- Computing Conformational Free Energies of iGluR Ligand-Binding Domains.- LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors.- Assaying the Energetics of NMDA Receptor Pore Opening.- Constructing a Rapid Solution Exchange System.- Assessing Effects of Ligand-Binding Mutations To AMPA And Kainate Receptor Kinetics.- Analysis of Whole-Cell NMDA Receptor Responses.- Expression, Purification and Crystallization of Full Length Ionotropic Glutamate Receptors.- Timing AMPA Receptor Activation with Laser-pulse Photolysis.- Current Recording and Kinetic Analyses for Single AMPA Receptors.- Extracting Rate Constants for NMDA Receptor Gating from One-channel Current Recordings.