Radoeva / van Dijk Apomixis

Methods and Protocols
Erscheinungsjahr 2025
ISBN: 978-1-0716-5001-1
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 302 Seiten

Reihe: Methods in Molecular Biology

This volume explores the latest research techniques (and reviews) used to study apomixis model species. The chapters in this book are organized into six parts: Part One introduces gametophytic apomixis, and Part Two focuses on the detection and characterization of apomixis. Part Three talks about transcriptomic profiling of apomictic and sexual species, and Part Four covers genetic analysis of apomixis. Part Five is dedicated to research on model apomictic systems such as dandelion ( ), ,  and ( and the final Part Six looks at protocols for engineering either individual components of apomixis, such as apomeiosis or a complete synthetic apomixis system. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, is a valuable reference for studying apomixis, and serves as an inspiration and foundation for designing new approaches and achieving significant breakthroughs in the field.

Chapter 17 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Radoeva, Tatyana

van Dijk, Peter J.

Inhaltsverzeichnis

Gametophytic Apomixis: An Introduction.- Flow Cytometric Seed Screening and Seed Genotyping: Methods for Detecting Reproductive Models in Flowering Plants.- Analysis of Gametophytic Apomixis Using Confocal Microscopy.- 3D Imaging, Segmentation, and Cell Annotation of the Ovule During Megaspore Mother Cell Differentiation in spp.- Imaging of Callose in Young Ovules of Diplosporous Species of the Asteraceae Family.- A Method for Detecting Parthenogenesis in Species.- Laser-Assisted Microdissection for Cell and Tissue-Type Specific Transcriptome and RNA-Seq Analysis.- Manual Isolation of Parthenogenetic Egg Cells Combined with Comparative Transcriptomics to Identify Parthenogenesis Genes in Tripsacum.- Gene Discovery in Reproduction: RNA-seq Analysis of isolated Pistils from Apomictic and Sexual Genetic Mapping in Apomicts.- Chromosome Preparation and Fluorescent In Situ Hybridization.- Targeted Mutagenesis in Natural Apomicts.- Map-Based Cloning of Apomixis Genes in Dandelion ( ).- A Dicotyledonous Model for Studying Aposporous, Autonomous Apomixis.- The Genus as a Model for Apomixis Research.- How Apomictic and Sexual Species Can Help Elucidate the Mechanisms Leading to Apospory.- Synthetic Apomeiosis in Tomato via the Mutagenesis of and to Engineer Engineering Synthetic Apomixis in Hybrid Rice.